Transcriptome analysis of differentially expressed genes in the red swamp crayfish Procambarus clarkii challenged with Aeromonas hydrophila
文献类型： 外文期刊
作者： Liu, Qiu-Ning 1 ; Huang, Long 1 ; Wang, Shu-Yu 1 ; Li, Yue-Tian 2 ; Tang, Ying-Yu 2 ; Zhang, Dai-Zhen 2 ; Tang, Bo-Pin 1 ;
作者机构： 1.Anhui Acad Agr Sci, Anhui Prov Key Lab Aquaculture Ampersand Stock En, Fishery Inst, Hefei, Peoples R China
2.Yancheng Teachers Univ, Jiangsu Synthet Innovat Ctr Coastal Bioagr, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Jiangsu Key Lab Bioresources Saline Soils,Sch Wet, Yancheng, Peoples R China
3.Wenzhou Med Univ, Sch Basic Med Sci, Dept Microbiol & Immunol, Wenzhou, Peoples R China
4.Shanghai Ocean Univ, Coll Aquaculture & Life Sci, Key Lab Freshwater Aquat Genet Resources, Minist Agr & Rural Affairs, Shanghai, Peoples R China
5.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou, Jiangsu, Peoples R China
关键词： Procambarus clarkii; Aeromonas hydrophila; Immune response; Transcriptome
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2021 年 119 卷
页码：
收录情况： SCI
摘要： As an important economic species in China, aquaculture of the crayfish Procambarus clarkii has suffered huge losses due to infection by pathogenic bacteria, mainly by Aeromonas hydrophila, which leads to high mortality and huge economic loss. To better understand the immune response of crayfish against bacterial infection, we compared and analyzed transcriptome data of hepatopancreatic tissue from P. clarkii that were either challenged with A. hydrophila or treated with PBS. After assembly and annotation of the data, 32,041 unigenes with an average length of 1512 base pairs were identified. Compared to control group, Differential gene expression (DEG) analysis revealed 608 DEGs were obtained, of which 274 unigenes were upregulated and 334 were downregulated in the A. hydrophila group. Furthermore, the expression levels of eight selected immune-related DEGs were validated by qRT-PCR, substantiating the reliability of RNA-seq results. This study not only provides effective data support for immune defense strategies of P. clarkii in response to bacterial infections, but also provides new information about the P. clarkii immune system and defense mechanisms, and a valuable basis for further studies to elucidate the molecular immune mechanisms of this species.
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