Molecular Cloning and Abiotic Stress Expression Analysis of GTPase Era Gene in Mulberry (Morus alba L.)
文献类型： 外文期刊
作者： Guo, P. 1 ; Hu, F. 2 ; Du, Q. -X. 1 ; Zheng, D. -Y. 1 ; Dominic, K. -T. 1 ; Ackah, M. 1 ; Li, Y. 1 ; Lin, Q. 3 ; Adolf, A. 1 ;
作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Nanxu Rd, Zhenjiang 212018, Jiangsu, Peoples R China
2.Anhui Acad Agr Sci, Plant Protect & Agroprod Safety Inst, Hefei 230031, Anhui, Peoples R China
3.Sericultural Res Inst, Nanning 530007, Guangxi Zhuang, Peoples R China
关键词： Morus alba; GTPase Era; molecular cloning; sequence analysis; expression patterns; abiotic stress
期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）
ISSN： 1021-4437
年卷期： 2021 年 68 卷 2 期
页码：
收录情况： SCI
摘要： Escherichia coli Ras-like protein (Era) forms a special GTPase family, which functions in the regulation of the cell cycle by coupling cell division with the growth rate. A cDNA sequence coding GTPase Era mRNA has been cloned from mulberry in the present report. Sequence analysis revealed that its ORF is 1275 bp in length, encoding 424 amino acid protein. The GTPase Era gene had an era domain that belonged to the FeoB_N superfamily. Phylogenetic analysis of the amino acid sequences encoded by the GTPase gene from different species reveals that Morus alba L. was closely connected to Morus notabilis, Ipomoea nill, Capsicum annuum, Solanum pennellii, Nicotiana sylvestris and Nicotiana tabacum. GTPase Era expression patterns treated under drought, low temperature, and salt stress treatment were investigated using quantitative PCR (RT-qPCR) in real-time. The expression level of mRNA showed a significant change in drought, cold, and salt stress treatment compared to the standard growth environment. The highest expression were observed on the third day for drought stress, on the sixth day for low temperature and the seventh day for the salt stress. These findings provide a more definite knowledge on the molecular basis of signal transduction in mulberry trees during stress responses.
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