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Analysis of histology and long noncoding RNAs involved in the rabbit hair follicle density using RNA sequencing

文献类型： 外文期刊

作者： Ding, Haisheng 1 ; Zhao, Huiling 1 ; Zhao, Xiaowei 1 ; Qi, Yunxia 1 ; Wang, Xiaofei 1 ; Huang, Dongwei 1 ;

作者机构： 1.Anhui Acad Agr Sci, Inst Anim Husb & Vet Med, Anhui Key Lab Livestock & Poultry Prod Safety Eng, Hefei 230031, Anhui, Peoples R China

关键词： Skin; Hair follicle density; Wool production; Histological analysis; LncRNA expression; RNA sequencing; Angora rabbit

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2021 年 22 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundHair follicle density influences wool fibre production, which is one of the most important traits of the Wan Strain Angora rabbit. However, molecular mechanisms regulating hair follicle density have remained elusive.ResultsIn this study, hair follicle density at different body sites of Wan Strain Angora rabbits with high and low wool production (HWP and LWP) was investigated by histological analysis. Haematoxylin-eosin staining showed a higher hair follicle density in the skin of the HWP rabbits. The long noncoding RNA (lncRNA) profile was investigated by RNA sequencing, and 50 and 38 differentially expressed (DE) lncRNAs and genes, respectively, were screened between the HWP and LWP groups. A gene ontology analysis revealed that phospholipid, lipid metabolic, apoptotic, lipid biosynthetic, and lipid and fatty acid transport processes were significantly enriched. Potential functional lncRNAs that regulate lipid metabolism, amino acid synthesis, as well as the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) and hedgehog signalling pathways, were identified. Consequently, five lncRNAs (LNC_002171, LNC_000797, LNC_005567, LNC_013595, and LNC_020367) were considered to be potential regulators of hair follicle density and development. Three DE lncRNAs and genes were validated by quantitative real-time polymerase chain reaction (q-PCR).ConclusionsLncRNA profiles provide information on lncRNA expression to improve the understanding of molecular mechanisms involved in the regulation of hair follicle density.

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