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InDel marker development and QTL analysis of agronomic traits in mung bean [Vigna radiate (L.) Wilczek]

文献类型： 外文期刊

作者： Ye, Weijun 1 ; Yang, Yong 1 ; Wang, Peiran 1 ; Zhang, Yin 1 ; Zhang, Liya 1 ; Tian, Dongfeng 1 ; Zhang, Lei 1 ; Zhang, Li 1 ;

作者机构： 1.Anhui Acad Agr Sci, Crop Res Inst, Hefei 230000, Anhui, Peoples R China

2.Anhui Prov Key Lab Crop Qual Improvement, Hefei 230000, Anhui, Peoples R China

关键词： Mung bean; InDel marker; Agronomic trait; Linkage map; QTL analysis

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2021 年 41 卷 10 期

页码：

收录情况： SCI

摘要： The stem color of young mung bean is a very useful tool in germplasm identification. Flowering time and plant height (PH) are known to be strongly correlated with crop adaption and yield. However, few studies have focused on elucidating the genetic mechanisms that regulate these five particular traits: young stem color (YSC), days to first flowering (DFF), days to maturity (DM), PH, and nodes on the main stem (NMS). In this study, a genetic linkage map for the F-2 population was constructed using 129 InDel markers that were developed based on the sequence variations between parents. A total of 14 QTLs related to YSC, DFF, DM, PH, and NMS were detected. These QTLs were distributed on six chromosomes (1, 3, 4, 6, 7, and 10), which individually accounted for 1.32 to 90.07% of the total phenotypic variation. Using a short and high-density linkage map for the F-3 population, six of the seven QTLs which clustered at two intervals on chromosomes 3 and 10 were detected again. Further analysis found that four QTLs between InDel markers R3-15 and R3-19 controlled DFF, DM, PH, and NMS, and each QTL accounted for a large percent of the total phenotypic variation. Analysis of two separated F-2:3 lines also found that the phenotype was highly corresponded to its genotype which was between R3-15 and R3-19. Phenotype and genotype analysis for 30 mung bean accessions showed that the major effect QTL qDFF3 was a key regulator for DFF. Using a map-based cloning method, the major effect QTL qYSC4 for YSC was mapped in a 347 Kb interval on chromosome 4. Candidate gene analysis showed that sequence variations and expression level differences existed in the predicted candidate gene between the parents. These results provide a theoretical basis for cloning these QTLs and marker-assisted selection.

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