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In vitro digestive properties and the bioactive effect of walnut green husk on human gut microbiota

文献类型: 外文期刊

作者: Zhao, Xiaolan 1 ; Ying, Jiabao 1 ; Wang, Zhuochen 3 ; Wang, Yulu 1 ; Li, Zhen 1 ; Gu, Tianyi 1 ; Liu, Shujun 1 ; Li, Yulong 1 ; Liu, Bing 2 ; Xin, Fengjiao 1 ; Wen, Boting 1 ;

作者机构: 1.Chinese Acad Agr Sci CAAS, Inst Food Sci & Technol, Lab Biomfg & Food Engn, Beijing, Peoples R China

2.Tianjin Univ Sci & Technol, Coll Food Sci & Engn, Key Lab Food Nutr & Safety, Tianjin, Peoples R China

3.Anhui Acad Agr Sci, Inst Agroprod Proc, Hefei, Peoples R China

4.Chinese Acad Agr Sci CAAS, Inst Agr Prod Proc & Nutr Hlth, Lab Biomfg & Food Engn, Cangzhou, Peoples R China

关键词: walnut green husk; in vitro digestion; in vitro fermentation; gut microbiota; metabolism

期刊名称:FRONTIERS IN MICROBIOLOGY ( 影响因子:4.5; 五年影响因子:5.2 )

ISSN:

年卷期: 2024 年 15 卷

页码:

收录情况: SCI

摘要: Introduction Walnut green husk (WGH) is a waste byproduct from walnut industry. However, it is not well-known about its bioactive effect on human gut health. Methods This study conducted in vitro digestion and fermentation experiments to study the bioactive effect of WGH. Results Microbial fermentation was the primary mechanism to efficiently release phenolics and flavonoids, resulting in more excellent antioxidant capacities (DPPH, ABTS, and FRAP assays), which reached a highest value with 14.82 +/- 0.01 mg VcE/g DW, 3.47 +/- 0.01 mmol TE/g DW, and 0.96 +/- 0.07 mmol FeSO47H(2)O/g DW, respectively. The surface microstructure of WGH became loose and fragmented after microbial fermentation. The analytical results of gut microbiota demonstrated that WGH could significantly increase the relative abundance of Proteobacteria in phylum level and Phascolarctobacterium in genus level while certain pro-inflammatory bacteria (such as Clostridium_sensu_stricto_1, Dorea, Alistipes, and Bilophila) was inhibited. Additionally, 1,373 differential metabolites were identified and enriched in 283 KEGG pathways. Of which some metabolites were significantly upregulated including ferulic acid, chlorogenic acid, umbelliferone, scopolin, muricholic acid, and so forth. Discussion These results indicated that WGH could have antioxidant and anti-inflammatory activities in the human gut, which could improve the economical value of WGH in the food industry.

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