Visual detection of fowl adenovirus serotype 4 via a portable CRISPR/Cas13a-based lateral flow assay
文献类型： 外文期刊
作者： Yin, Dongdong 1 ; Yin, Lei 1 ; Wang, Jieru 1 ; Dai, Yin 1 ; Shen, Xuehuai 1 ; Zhao, Ruihong 1 ; Qi, Kezong 2 ; Pan, Xiaocheng 1 ;
作者机构： 1.Anhui Acad Agr Sci, Inst Anim Husb & Vet Sci, Livestock & Poultry Epidem Dis Res Ctr Anhui Prov, Anhui Prov Key Lab Livestock & Poultry Prod Safety, Hefei, Peoples R China
2.Anhui Agr Univ, Coll Anim Sci andTechnol, Anhui Prov Key Lab Vet Pathobiol & Dis Control, Hefei, Peoples R China
关键词： Fowl adenovirus serotype 4; RAA; CRISPR/Cas13a; SHERLOCK; collateral cleavage; lateral flow detection
期刊名称：AVIAN PATHOLOGY （ 影响因子：2.8； 五年影响因子：3.2 ）
ISSN： 0307-9457
年卷期： 2023 年 52 卷 6 期
页码：
收录情况： SCI
摘要： The widespread occurrence of fowl adenovirus serotype 4 (FAdV-4)-induced hepatitis-hydropericardium syndrome (HHS) has led to significant economic losses for the poultry industry. A sensitive, accurate, and practical FAdV-4 diagnostic approach is urgently required to limit the incidence of the disease. In the present study, a practical method for detecting FAdV-4 was developed using the CRISPR/Cas13a system and recombinase-aided amplification. The approach was based on 37 degrees C isothermal detection with visible results being achieved. The detection limit of the target gene with this approach was only 10(1) copies/mu l, making it very sensitive and specific. Clinical samples fared well when tested with the Cas13a detection method. For identifying FAdV-4, this novel detection approach was found to be sensitive, specific, and effective.
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