Alterations in bone marrow microRNA expression profiles on infection with avian pathogenic Escherichia coli
文献类型: 外文期刊
作者: Yin, Lei 1 ; Shen, Xuehuai 1 ; Zhang, Danjun 1 ; Zhao, Ruihong 1 ; Hou, Hongyan 1 ; Hu, Xiaomiao 1 ; Wang, Jieru 1 ; Dai, Yin 1 ; Pan, Xiaocheng 1 ; Qi, Kezong 3 ;
作者机构: 1.Anhui Acad Agr Sci, Inst Anim Husb & Vet Sci, Livestock & Poultry Epidem Dis Res Ctr Anhui Prov, Hefei 230031, Anhui, Peoples R China
2.Anhui Prov Key Lab Livestock & Poultry Prod Safety, Hefei 230031, Anhui, Peoples R China
3.Anhui Agr Univ, Coll Anim Sci & Technol, Anhui Prov Key Lab Vet Pathobiol & Dis Control, Hefei 230036, Peoples R China
关键词: Avian pathogenic Escherichia coli; Bone marrow; microRNA; Gene regulation; Immune response
期刊名称:RESEARCH IN VETERINARY SCIENCE ( 影响因子:2.554; 五年影响因子:2.564 )
ISSN: 0034-5288
年卷期: 2022 年 150 卷
页码:
收录情况: SCI
摘要: Avian pathogenic Escherichia coli (APEC) is one of the most common avian bacterial diseases globally. The bone marrow is a reservoir of immature immune cells. To elucidate the role of bone marrow microRNAs (miRNAs) in regulating the host response to APEC infection, we performed miRNA-seq to investigate alterations in the expression of bone marrow miRNAs in three groups of specific pathogen-free chickens: non-challenged (NC) and challenged with APEC for 12 h (C12) and 24 h (C24). Twenty and 19 differentially expressed miRNAs (fold change > 2, P < 0.01) were identified on comparing the NC and C12 and the NC and C24 groups, respectively. On functional annotation analysis of target genes of differentially expressed miRNAs, we found that the gene ontology term "immune system process " was significantly enriched at both 12 h and 24 h; moreover, several important signaling pathways were triggered in response to APEC infection, such as MAPK, cGMP-PKG, Notch, and cAMP pathways. In addition, we performed reverse transcription quantitative real-time PCR (qRT-PCR) to validate the differential expression of miRNAs. qRT-PCR data were similar to the sequencing data. On con-structing an miRNA-target gene network, gga-miR-2127, gga-miR-6643-5p, and gga-miR-6567-3p were found to potentially play a vital role in the immune process. Overall, our findings provide deeper insights into miRNA transcriptome changes involved in the immune response of the chicken bone marrow to APEC infection.
- 相关文献
作者其他论文 更多>>
-
Genomic evolution and insights into agronomic trait innovations of Sesamum species
作者:Miao, Hongmei;Wei, Shuangling;Zheng, Yongzhan;Duan, Yinghui;Li, Chun;Zhao, Ruihong;Mu, Cong;Tian, Qiuzhen;Gao, Tongmei;Zhang, Haiyang;Wang, Lei;Sun, Yamin;Lin, Wenchao;Xiong, Songjin;Qu, Lingbo;Wang, Xuede;Liu, Hongyan;Le, Meiwang;Wang, Qiang
关键词:Sesamum; genome evolution; structural variation; plant architecture; Fusarium wilt disease; oil content
-
IFI16 plays a critical role in avian reovirus induced cellular immunosuppression and suppresses virus replication
作者:Zhang, Chengcheng;Zhang, Qingqing;Zhang, Xiaorong;Wu, Yantao;Zhang, Chengcheng;Zhang, Qingqing;Zhang, Xiaorong;Wu, Yantao;Li, Wei;Hu, Xiaomiao
关键词:Avian reovirus; p17; IFI16; IFN-b; protein interaction
-
High-Performance Detection of Mycobacterium bovis in Milk Using Recombinase-Aided Amplification-Clustered Regularly Interspaced Short Palindromic Repeat-Cas13a-Lateral Flow Detection
作者:Wang, Jieru;Zeng, Xiaoyu;Yin, Dongdong;Gou, Jiaojiao;Pan, Xiaocheng;Wang, Nan;Xu, Lei;Cheng, Junsheng;Zhang, Xiaoqian;Zhang, Yinghui;Zhu, Xiaojie
关键词:milk; food safety; bovine tuberculosis; CRISPR-Cas13a; rapid detection
-
Isolation, characterization, evaluation of pathogenicity, and immunomodulation through interferon production of duck adenovirus type-3 (DAdV-3)
作者:Tan, Yang;Rahim, Muhammad Ajwad;Xin, Huang;Zhou, Yuhang;Hu, Xuerui;Chen, Fangfang;Raheem, Muhammad Akmal;Raheem, Muhammad Akmal;Dai, Yin;Ataya, Farid Shokry
关键词:DAdV-3; separation; pathogenicity; interferon; cytokine
-
Antiviral activity of luteolin against porcine epidemic diarrhea virus in silico and in vitro
作者:Wang, Jieru;Zeng, Xiaoyu;Gou, Jiaojiao;Yin, Dongdong;Yin, Lei;Shen, Xuehuai;Dai, Yin;Pan, Xiaocheng;Zhu, Xiaojie
关键词:PEDV; Luteolin; Porcine ACE2; Spike; Mpro; Pro-inflammatory cytokine; Drug resistant mutant
-
Effects of adding antibiotics to an inactivated oil-adjuvant avian influenza vaccine on vaccine characteristics and chick health
作者:Shen, Xuehuai;Zhao, Ruihong;Yin, Lei;Yin, Dongdong;Dai, Yin;Hou, Hongyan;Wang, Jieru;Hu, Xiaomiao;Pan, Xiaocheng;Zhang, Danjun;Liu, Wei;Zhan, Kai;Shen, Xuehuai;Liu, Yongjie;Zhang, Anyun
关键词:avian influenza vaccines; antibiotic; immunization effect; drug concentration; chick health
-
Altered Proteomic Profile of Exosomes Secreted from Vero Cells Infected with Porcine Epidemic Diarrhea Virus
作者:Shen, Xuehuai;Yin, Lei;Xu, Shuangshuang;Wang, Jieru;Yin, Dongdong;Zhao, Ruihong;Pan, Xiaocheng;Dai, Yin;Hou, Hongyan;Zhou, Xueli;Hu, Xiaomiao;Shen, Xuehuai;Yin, Lei;Xu, Shuangshuang;Wang, Jieru;Yin, Dongdong;Zhao, Ruihong;Pan, Xiaocheng;Dai, Yin;Hou, Hongyan;Zhou, Xueli;Hu, Xiaomiao;Shen, Xuehuai
关键词:PEDV; Vero cells; exosomes; proteomics; cellular regulatory pathway