Visual detection and differentiation of porcine epidemic diarrhea virus wild-type strains and attenuated vaccine strains using CRISPR/Cas13a-based lateral flow strip
文献类型： 外文期刊
作者： Yin, Dongdong 1 ; Yin, Lei 1 ; Guo, Hao 2 ; Wang, Jieru 1 ; Shen, Xuehuai 1 ; Zhao, Ruihong 1 ; Pan, Xiaocheng 1 ; Dai, Yin 1 ;
作者机构： 1.Anhui Acad Agr Sci, Inst Anim Husb & Vet Sci, Livestock & Poultry Epidem Dis Res Ctr Anhui Prov, Anhui Prov Key Lab Livestock & Poultry Prod Safety, Hefei, Peoples R China
2.Anim Hlth Supervis Inst, Feixi Cty Agr & Rural Bur, Hefei, Peoples R China
关键词： PEDV wild-type strain; attenuated vaccine strain; CRISPR; Cas13a; RAA; lateral flow detection
期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：6.073； 五年影响因子：6.34 ）
ISSN： 2235-2988
年卷期： 2022 年 12 卷
页码：
收录情况： SCI
摘要： Porcine epidemic diarrhea virus (PEDV) is an enteric coronavirus that causes acute watery diarrhea and vomiting in unweaned piglets. Infections result in high mortality and serious economic losses to the swine industry. PEDV attenuated vaccine does not completely protect against all mutant wild-type strains, and PEDV infection can periodically occur. A sensitive, accurate, and simple detection method for PEDV is needed to reduce the occurrence of the disease. In this study, the CRISPR/Cas13a system was combined with recombinase aided amplification to develop a rapid diagnostic method to distinguish PEDV wild-type strains from attenuated vaccine strains. The method is based on isothermal detection at 37 degrees C. The results are used for visual readout. The assay had high sensitivity and specificity, with a detection limit of 10(1) copies/mu L for the gene of interest, and no cross-reactivity with other pathogens. The Cas13a detection worked well with clinical samples. This visual, sensitive, and specific nucleic acid detection method based on CRISPR/Cas13a should be a powerful tool for detecting PEDV.
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