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A fluorescence-based Coomassie Blue protocol for two-dimensional gel-based proteomics

文献类型: 外文期刊

作者: Yang, Yongxin 1 ; Wang, Jiaqi 1 ; Bu, Dengpan 1 ; Zhang, Leying 1 ; Li, Shanshan 1 ; Zhou, Lingyun 1 ; Wei, Hongyang 1 ;

作者机构: 1.Chinese Acad Agr Sci, State Key Lab Anim Nutr, Inst Anim Sci, Beijing 100193, Peoples R China

2.Anhui Acad Agr Sci, Inst Anim Sci, Hefei 230031, Peoples R China

期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )

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收录情况: SCI

摘要: A sensitive and convenient "visible SYPRO" staining protocol was developed for visualizing proteins after SDS-PAGE. Gels were sensitized with SYPRO Ruby and then stained with the Coomassie Brilliant Blue G-250 protocol (Blue Silver). This combined protocol had similar or better linearity than staining with only SYPRO Ruby or Blue Silver, respectively. In addition, this method was more sensitive than that of Blue Silver, simpler than that of SYPRO Ruby, and compatible with subsequent mass spectrometry analysis.

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