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Marker-assisted breeding of rice lines special for rice noodle-making by pyramiding the Wx(g2) and ALK(GC) genes

文献类型： 外文期刊

作者： Teng, Bin 1 ; Song, Fengshun 1 ; Gan, Quan 1 ; Lin, Cuixiang 1 ; Ni, Dahu 1 ;

作者机构： 1.Anhui Acad Agr Sci, Inst Rice Res, Anhui Prov Key Lab Rice Genet & Breeding, Hefei 230031, Peoples R China

关键词： Rice; Wx(g2) gene; ALK(GC) gene; Rice noodle; Marker-assisted selection

期刊名称：EUPHYTICA （ 影响因子：1.9； 五年影响因子：2.1 ）

ISSN： 0014-2336

年卷期： 2023 年 219 卷 2 期

页码：

收录情况： SCI

摘要： Rice noodles are a popular food widely consumed in South Asia. Up to now the breeding progress in the development of rice varieties used for rice noodle-making by conventional breeding methods is very limited, owing to the complex nature of grain quality traits. Fortunately, two functional genes, i.e., Wx(g2) (showing high amylose and hard gel consistency) and ALK(GC) (associated with intermediate high gelatinization temperature), have been identified to have desired starch quality phenotypes for rice noodle-making. This research was carried out to transfer the two major dominant genes from two rice donors HJX74 (carrying Wx(g2)) and ZX14 (carrying ALK(GC)) into new rice lines through marker-assisted breeding. Based on the functional mutations of Wx(g2) and ALK(GC), two sets of allele-specific PCR markers were developed. An intercross was made between HJX74 and ZX14 to develop a F-2 population in which marker-assisted selection was applied to select individuals possessing both Wx(g2) and ALK(GC) in homozygous condition. According to the marker genotypes, five homozygous lines were finally selected from 60 F-2 progenies. The two-gene pyramided lines were selfed to generate F-3 generation. Several promising F-3 lines with fine agro-morphological traits were identified and then advanced to F-4 generation for further evaluation of grain quality traits. All F-4 selected lines exhibited desired starch properties required for rice noodles, demonstrating that marker-assisted pyramiding of Wx(g2) and ALK(GC) is an essential approach to develop the rice lines suitable for rice noodle-making.

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