文献类型： 外文期刊

作者： Xu Jia-Ping ² ; Chen Ke-Ping ¹ ; Liu Ming-Hui ³ ; Yao Qin ¹ ; Gao Gui-Tian ¹ ; Zhao Yuan ¹ ;

作者机构： 1.Jiangsu Univ, Inst Life Sci, Zhenjiang 212013, Peoples R China

2.Anhui Agr Univ, Dept Life Sci, Hefei 230036, Peoples R China

3.Anhui Acad Agr Sci, Inst Sericultural, Hefei 230061, Peoples R China

关键词： BmNPV;Resistance;Bms3a;fluorescent differential display

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2008 年 7 卷 19 期

页码：

收录情况： SCI

摘要： Using fluorescent differential display (FDD) technique, we analyzed the differential expression of genes related to BmNPV resistance in highly resistant silkworm strain NB, highly susceptible silkworm strain 306 and near isogenic line 306NNZZ. Based on the differential display bands, a 609 bp fragment named C18(609) was cloned and confirmed by Northern blot hybridization. The sequence was then electric extended by identified in NCBI ESTs. A novel gene was characterized and revealed to encode a putative BmS3a protein. Becasue it has high homology to some insect S3a protein, it was named s3a. S3a protein have been known to play crucial roles in protein synthesis, and is related to apoptosis. It is differentially expressed in silkworm high resistance strain, high susceptible strain and BmNPV treated silkworms. Therefore, it is conceivable that BmS3a is involved in silkworm BmNPV resistance.