文献类型： 外文期刊

作者： Li Yan-he ¹ ; Zheng Fang-liang ² ; Chen Hong-quan ¹ ; Wang Han-zhong ² ; Wang Liu-quan ³ ; Xu Di-ping ⁴ ;

作者机构： 1.Anhui Agr Univ, Hefei 230036, Peoples R China

2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China

3.Anhui Acad Agr Sci, Inst Fisheries, Hefei 230031, Peoples R China

4.Hubei Acad Agr Sci, Huibei Key Lab Anim Embryo & Mol Breeding, Wuhan 430064, Peoples R China

关键词： prophenoloxidase cDNA;Procambarus clarkii;nucleotide sequence;copper-binding sites;intron

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2009 年 8 卷 3 期

页码：

收录情况： SCI

摘要： The full-length cDNA sequence of prophenoloxidase was obtained through RACE technology. The complete cDNA sequence is 3 721-bp long, containing an open reading frame (ORF) of 1 881 bp, a 154-bp 5'-untranslated region, and a 1 686-bp 3'-untranslated region with three potential functional poly(A) signals (AATAAA). The molecular mass of the deduced amino acid sequence (627 aa) was 72.3 kDa with an estimated pI of 5.88. It contained putative copper-binding sites (copper A: 131, 135, 167 and copper B: 301, 305, 341), and a tentative complement-like motif (GCGWPDHL). Eight potential N-linked glycosylation sites were predicted to be present in P. clarkii prophenoloxidase. Similar to those in other arthropod prophenoloxidases reported so far, no signal peptide was detected in the crayfish prophenoloxidase. The phylogenetic trees confirmed that P. clarkii prophenoloxidase was most closely related to that of freshwater crayfish P. leniusculus and more closely related to other crustacean prophenoloxidases from shrimp, prawn, and lobster than to the insect prophenoloxidases. Besides, two putative introns were found in this sequence of genomic DNA.