文献类型： 外文期刊
作者： Chen, Lei 2 ; Su, Jianyu 1 ; Zhang, Xiangzhai 3 ; Li, Lin 1 ; He, Xiaowei 1 ;
作者机构： 1.S China Univ Technol, Sch Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China
2.Anhui Acad Agr Sci, Inst Agroprod Proc, Hefei 230031, Peoples R China
3.Hebei Normal Univ Sci & Technol, Key Lab Hebei Prov Prevent Vet Med, Qinhuangdao 066004, Peoples R China
关键词： Sulfadimethoxine (SDM);sulfasulfonamides (SN);sulfadiazine (SD);sulfamethazine (SM2);sulfamethoxypyrazine (SMP);sulfalene (SMZ);sulfaquinoxaline (SQX);rabbit anti-SDM antibodies;soy 11S globulin;SDM-beta-amylase
期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）
ISSN： 1684-5315
年卷期： 2011 年 10 卷 48 期
页码：
收录情况： SCI
摘要： Sulfadimethoxine (SDM) is an antibiotic commonly added to animal feeds. Anti-SDM antibodies are useful for the detection of residual SDM in foods, feeds and biological fluids by ELISA. In this study, we show that SDM is immunogenic in rabbits when it is conjugated with soy 11S globulin or with beta-amylase. Rabbit anti-SDM antibodies obtained by immunization with SDM-11S and SDM-beta-amylase displayed low cross-reactivity against other sulfonamides, including sulfamethazine (SM2), sulfasulfonamides (SN), sulfadiazine (SD), sulfamethoxypyrazine (SMP), sulfalene (SMZ), sulfaquinoxaline (SQX). Thus, soy 11S globulin and beta-amylase are suitable carriers for the induction of anti-SDM antibodies and have the advantage of being cheaper that BSA.
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