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Detection of Viable Cronobacter spp. (Enterobacter sakazakii) by One-Step RT-PCR in Dry Aquatic Product

文献类型： 外文期刊

作者： Ye, Yingwang 1 ; Wu, Qingping 1 ; Zhang, Jumei 1 ; Jiang, He 3 ; Hu, Wang 3 ;

作者机构： 1.Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbiol Culture Collect, Guangzhou 510070, Guangdong, Peoples R China

2.Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China

3.Anhui Acad Agr Sci, Inst Fishery, Hefei 230039, Peoples R China

关键词： Cronobacter;DFI medium;FDA method;one-step RT-PCR

期刊名称：JOURNAL OF FOOD SCIENCE （ 影响因子：3.167； 五年影响因子：3.376 ）

ISSN： 0022-1147

年卷期： 2012 年 77 卷 11 期

页码：

收录情况： SCI

摘要： Cronobacter are opportunistic food-borne pathogens associated with meningitis, sepsis, and necrotizing enterocolitis. Little attempt has focused on detection of viable cell of Cronobacter spp. in dry aquatic products, which were frequently used for raw materials of infant foods due to high nutrition. In this paper, one-step reverse transcription polymerase chain reaction (RT-PCR) was developed for detection of viable Cronobacter spp. in dry aquatic products. Specificity test indicated that clearly expected amplicon in size 469bp was amplified from RNA of Cronobacter, but not from RNA of negative controls and DNA of Cronobacter strains. The sensitivity was 10(4) CFU/mL of Cronobacter strain in artificially fish meal samples and 10(1) CFU/mL of Cronobacter after 10-h enrichment. In a total of 81 dry aquatic products, 9.8%, 8.6%, and 9.8% of samples were found to be positive for Cronobacter by one-step RT-PCR, U. S. Food and Drug Administration method, and Druggan-Forsythe-Iversen medium, respectively. The results clearly indicated that one-step RT-PCR could avoid the interference of residual DNA of Cronobacter in food samples and be used to specifically detect viable Cronobacter spp. for large-scale monitoring of food samples.

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