文献类型： 外文期刊

作者： Xu, Wenjing ¹ ; Shi, Lingling ¹ ; Chan, Onchim ¹ ; Li, Jiao ¹ ; Casper, Peter ⁵ ; Zou, Xiaoming ⁶ ;

作者机构： 1.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Key Lab Trop Forest Ecol, Kunming, Yunnan, Peoples R China

2.Univ Chinese Acad Sci, Beijing, Peoples R China

3.Anhui Acad Agr Sci, Inst Agr Engn, Hefei, Anhui, Peoples R China

4.Yunnan Univ, Fac Life Sci, Dept Biol, Kunming, Yunnan, Peoples R China

5.Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Expt Limnol, Stechlin, Germany

6.Nanjing Forestry Univ, Coll Forest Resources & Environm, Nanjing, Jiangsu, Peoples R China

7.Univ Puerto Rico, Inst Trop Ecosyst Studies, San Juan, PR 00936 USA

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2013 年 8 卷 12 期

页码：

收录情况： SCI

摘要： Although bacteria and fungi are well-known to be decomposers of leaf litter, few studies have examined their compositions and diversities during the decomposition process in tropical stream water. Xishuangbanna is a tropical region preserving one of the highest floristic diversity areas in China. In this study, leaf litter of four dominant plant species in Xishuangbanna was incubated in stream water for 42 days during which samples were taken regularly. Following DNA extraction, PCR-DGGE (denaturing gradient gel electrophoresis) and clone-sequencing analyses were performed using bacterial and fungal specific primers. Leaf species have slightly influences on bacterial community rather than fungal community. The richness and diversity of bacteria was higher than that of fungi, which increased towards the end of the 42-day-incubation. The bacterial community was initially more specific upon the type of leaves and gradually became similar at the later stage of decomposition with alpha-proteobacteria as major component. Sequences affiliated to methanotrophs were obtained that indicates potentially occurrence of methane oxidation and methanogenesis. For the fungal community, sequences affiliated to Aspergillus were predominant at the beginning and then shifted to Pleosporales. Our results suggest that the microorganisms colonizing leaf biofilm in tropical stream water were mostly generalists that could exploit the resources of leaves of various species equally well.