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Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)

文献类型： 外文期刊

作者： Chen, Hao-Liang 1 ; Zhangl, Hong-Yu 1 ; Throne, James E. 3 ; Zhu, Kun Yan 4 ;

作者机构： 1.Huazhong Agr Univ, Hubei Insect Resources Utilizat & Sustainable Pes, State Key Lab Agr Microbiol, Inst Urban & Hort Pests,Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China

2.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei, Peoples R China

3.ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS USA

4.Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA

关键词： ectoparasitoid;gene expression;Habrobracon hebetor;heat shock protein 70;phylogenetic analysis;quantitative RT-PCR

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2014 年 21 卷 4 期

页码：

收录情况： SCI

摘要： Heat shock proteins (HSPs) are known as chaperones that help with folding of other proteins when cells are under environmental stresses. The upregulation of HSPs is essential for cold survival during insect diapause. The ectoparasitoid Habrobraconhebetor, a potential biological control agent, can enter reproductive diapause when reared at low temperature and short photoperiod. However, the expression of HSPs during diapause of H. hebetor has not been studied. In this study, we sequenced and characterized the full-length complementary DNAs of three Hsp70 genes (HhHsp70I, HhHsp70II and HhHsp70III) from H. hebetor. Their deduced amino acid sequences showed more than 80% identities to their counterparts from other insect species. However, the multiple sequence alignment among the three deduced amino acid sequences of HhHsp70s showed only 46% identities. A phylogenetic analysis of the three HhHsp70s and all other known Hsp70 sequences from Hymenoptera clustered all the Hsp70s into four groups, and the three HhHsp70s were distributed into three different groups. Real-time quantitative polymerase chain reaction analysis showed that the expression of the three HhHsp70 genes in H. hebetor reared at different conditions was quite different. HhHsp70I showed higher relative expression when H. hebetor were reared at 27.5 degrees C than at two lower temperatures (17.5 degrees C and 20 degrees C) regardless of the photoperiod, whereas HhHsp70II showed higher expression when H. hebetor were reared at 20 degrees C and 10 : 14 L : D than when reared at 17.5 degrees C and either 16: 8 L: D or 10 : 14 L : D. In contrast, HhHSP70III was expressed at similar levels regardless of the rearing conditions. These results may suggest functional differences among the three HhHsp70 genes in H. hebetor.

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