文献类型： 外文期刊
作者： Duan, Y. B. 1 ; Zhao, F. L. 1 ; Chen, H. D. 1 ; Li, H. 2 ; Ni, D. H. 2 ; Wei, P. C. 2 ; Sheng, W. 1 ; Teng, J. T. 1 ; Zhang, 1 ;
作者机构： 1.Huaibei Normal Univ, Coll Life Sci, Key Lab Resource Plant Biol Anhui Prov, Huaibei, Peoples R China
2.Anhui Acad Agr Sci, Rice Res Inst, Key Lab Rice Genet Breeding Anhui Prov, Hefei, Peoples R China
关键词： DNA extraction;Half-seed;Polymerase chain reaction;Pre-germination genotyping;Oryza sativa L.
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2015 年 14 卷 2 期
页码：
收录情况： SCI
摘要： Genotyping is a critical step for molecular marker-assisted selection in rice. Rice genomic DNA samples for genotyping are typically isolated from living tissues such as seedlings. This requires the germination of all candidate seeds and extraction of DNA from the seedlings. Currently, an ideal individual is selected from a very large number of plants, which is time-and labor-consuming, requiring several transplantations of materials and sampling processes. In this study, we developed a simplified genomic DNA extraction protocol in rice by using amylase to treat half-seeds. The yields of genomic DNA from a half-seed of Indica and Japonica rice were greater than 203.8 +/- 32.5 and 143.2 +/- 25.5 ng, respectively, and the 260/280 nm absorbance ratio was 1.75-2.10. The DNA was confirmed to be sufficient for polymerase chain reaction amplification and can be used in a marker-assisted selection program.
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