Detection of a mutation at codon 43 of the rpsL gene in Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae by PCR-RFLP
文献类型： 外文期刊
作者： Zhang, Y. 1 ; Yang, X. 1 ; Zhou, F. Y. 1 ; Zhang, A. F. 1 ; Zhu, X. F. 2 ; Chen, Y. 1 ; Zhou, M. G. 2 ; Gao, T. C. 1 ;
作者机构： 1.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei, Peoples R China
2.Nanjing Agr Univ, Coll Crop Protect, Nanjing, Jiangsu, Peoples R China
关键词： Molecular diagnosis of PCR-RFLP;Point mutation;Ribosomal protein S12 (rpsL) gene;X. oryzae pv. oryzae;Xanthomonas oryzae pv. oryzicola
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2015 年 14 卷 4 期
页码：
收录情况： SCI
摘要： The aim of this study was to develop a method to detect a point mutation in the ribosomal S12 protein (rpsL) gene in streptomycin-resistant strains of Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to detect a point mutation in codon 43 of the rpsL gene in X. oryzae pv. oryzicola and X. oryzae pv. oryzae. The 304-bp PCR product from the rpsL gene was digested by MboII to form two fragments (201 and 103 bp) if there was a mutation at codon 43, or three fragments (146, 103, and 55 bp) if there was no mutation. Compared with the results from nucleotide sequencing, the PCR-RFLP method was accurate in detecting the point mutation at codon 43 of the rpsL gene in streptomycin-resistant strains of X. oryzae pv. oryzicola and X. oryzae pv. oryzae. These results indicate that the PCR-RFLP is a simple, rapid and reliable method for detecting the point mutation at codon 43 of the rpsL gene.
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