您好，欢迎访问安徽省农业科学院 机构知识库！

Using Next-Generation Sequencing to Detect Differential Expression Genes in Bradysia odoriphaga after Exposure to Insecticides

文献类型： 外文期刊

作者： Chen, Haoliang 1 ; Lin, Lulu 1 ; Ali, Farman 1 ; Xie, Minghui 1 ; Zhang, Guangling 1 ; Su, Weihua 1 ;

作者机构： 1.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei 230031, Anhui, Peoples R China

2.Abdul Wali Khan Univ, Dept Agr, Mardan 23200, Khyber Pakhtunk, Pakistan

关键词： next-generation sequencing;Bradysia odoriphaga;differential expression genes;insecticide

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2017 年 18 卷 11 期

页码：

收录情况： SCI

摘要： Bradysia odoriphaga (Diptera: Sciaridae) is the most important pest of Chinese chive. Insecticides are used widely and frequently to control B. odoriphaga in China. However, the performance of the insecticides chlorpyrifos and clothianidin in controlling the Chinese chive maggot is quite different. Using next generation sequencing technology, different expression unigenes (DEUs) in B. odoriphaga were detected after treatment with chlorpyrifos and clothianidin for 6 and 48 h in comparison with control. The number of DEUs ranged between 703 and 1161 after insecticide treatment. In these DEUs, 370-863 unigenes can be classified into 41-46 categories of gene ontology (GO), and 354-658 DEUs can be mapped into 987-1623 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The expressions of DEUs related to insecticide-metabolism-related genes were analyzed. The cytochrome P450-like unigene group was the largest group in DEUs. Most glutathione S-transferase-like unigenes were down-regulated and most sodium channel-like unigenes were up-regulated after insecticide treatment. Finally, 14 insecticide-metabolism-related unigenes were chosen to confirm the relative expression in each treatment by quantitative Real Time Polymerase Chain Reaction (qRT-PCR). The results of qRT-PCR and RNA Sequencing (RNA-Seq) are fairly well-established. Our results demonstrate that a next-generation sequencing tool facilitates the identification of insecticide-metabolism-related genes and the illustration of the insecticide mechanisms of chlorpyrifos and clothianidin.

  • 相关文献

[1]De novo sequencing and characterization of the Bradysia odoriphaga (Diptera: Sciaridae) larval transcriptome. Chen, Haoliang,Lin, Lulu,Xie, Minghui,Zhang, Guangling,Su, Weihua.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台