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Repeated-batch fermentation by immobilization of Clostridium beijerinckii NCIMB 8052 in a fibrous bed bioreactor for ABE (acetone-butanol-ethanol) production

文献类型: 外文期刊

作者: Liu, Jun 1 ; Liu, Zuojun 3 ; Guo, Ting 1 ;

作者机构: 1.Cent South Univ Forestry & Technol, Natl Engn Lab Rice & By Prod Deep Proc, Shaoshan Nan Rd 498, Changsha 410004, Hunan, Peoples R China

2.Cent South Univ Forestry & Technol, Coll Food Sci & Technol, Shaoshan Nan Rd 498, Changsha 410004, Hunan, Peoples R China

3.Anhui Acad Agr Sci, Agr Engn Res Inst, 40 South Nongke Rd, Hefei 230001, Anhui, Peoples R China

期刊名称:JOURNAL OF RENEWABLE AND SUSTAINABLE ENERGY ( 影响因子:2.219; 五年影响因子:2.004 )

ISSN: 1941-7012

年卷期: 2018 年 10 卷 1 期

页码:

收录情况: SCI

摘要: In this study, a fibrous bed bioreactor (FBB) system was used to produce ABE (acetone-butanol-ethanol) by immobilized cells of Clostridium beijerinckii NCIMB 8052. To obtain the maximum ABE productivity and yield and maintain the stability of a repeated-batch fermentation process in the FBB system, the optimal dilution rate (0.17 min(-1)) and initial glucose concentration (18 g/l) in the bioreactor were determined. When the repeated-batch fermentation was maintained at 11 cycles under the optimal conditions, an average of 4.9 g/l butanol (maximum 5.3 g/l) was achieved with an average solvent productivity of 1.0 g/l/h and a yield of 0.41 g/g (maxima of 1.1 g/l/h and 0.43 g/g, respectively). However, the solvent productivity and yield in batch fermentation of free cells were just 0.29 g/l/h and 0.36 g/g, respectively. In addition, scanning electron micrographs demonstrated that C. beijerinckii cells can be adsorbed onto a cotton towel and they exhibited a significantly different morphology compared to immobilized Clostridium acetobutylicum. Published by AIP Publishing.

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