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Identification of VcRBOH genes in blueberry and functional characterization of VcRBOHF in plant defense

文献类型： 外文期刊

作者： Song, Zhiqiang 1 ; Chen, Chao 3 ; Duan, Hua 1 ; Yu, Ting 1 ; Zhang, Yaqian 1 ; Wei, Yuneng 1 ; Xu, Decong 1 ; Liu, Dong 2 ;

作者机构： 1.Hefei Normal Univ, Anhui Blueberry Engn Technol Res Ctr, Collaborat Technol Serv Ctr, Sch Biol & Food Engn,Anhui Green Food Rural Revita, Hefei 230601, Peoples R China

2.Anqing Vocat & Tech Coll, Sch Agr Forestry & Clothing, Anqing 246003, Peoples R China

3.Anhui Acad Agr Sci, Inst Ind Crops, Hefei 230031, Peoples R China

关键词： Reactive oxygen species (ROS); RBOH family; Blueberry; Disease resistance

期刊名称：BMC GENOMICS （ 影响因子：3.7； 五年影响因子：4.2 ）

ISSN： 1471-2164

年卷期： 2025 年 26 卷 1 期

页码：

收录情况： SCI

摘要： Reactive oxygen species (ROS) serve as signal molecules in plant defense responses, and the respiratory burst oxidase homolog (RBOH) enzyme plays a crucial role in their production. Although numerous RBOH family members have been identified in various plants, little is known about the RBOH genes in blueberries. In this study, we identified six VcRBOH genes from the blueberry genome. Phylogenetic analysis revealed that these VcRBOH genes can be classified into three subgroups. Conserved domain and motif analysis demonstrated high sequence similarity among VcRBOH proteins. Analysis of cis-acting elements suggested that VcRBOH genes may be involved in stress, light, and phytohormone responsiveness. Based on transcriptome data, we observed low expression levels of VcRBOHB, VcRBOHC, and VcRBOHE during the flower_at_anthesis stage. In contrast, VcRBOHA and VcRBOHD showed relatively high expression levels in various tissues. The reverse-transcription quantitative PCR (RT-qPCR) analysis indicated rapid induction of VcRBOHF by flg22 and chitin treatments. Notably, overexpression of VcRBOHF in Arabidopsis promoted PTI responses, including increased expression of marker genes, ROS production, and callose deposition. Moreover, the overexpression of VcRBOHF resulted in enhanced disease resistance against Pseudomonas syringae pv. tomato (Pst) DC3000 infection. These findings provide valuable insights into the roles of VcRBOHF genes in plant defense responses and lay the groundwork for a more comprehensive understanding of the molecular mechanisms underpinning blueberry disease resistance.

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