The Golgi-localized transporter OsPML3 is involved in manganese homeostasis and complex N-glycan synthesis in rice
文献类型： 外文期刊
作者： Xu, Ending 1 ; Wu, Mengyao 1 ; Liu, Yuanyuan 1 ; Tai, Yingping 1 ; Zha, Wenjuan 1 ; Gong, Changyi 1 ; Zou, Yu 2 ; Zhang, Peijiang 2 ; Zhang, Wei 1 ; Chen, Xi 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Dept Biochem & Mol Biol, Nanjing 210095, Jiangsu, Peoples R China
2.Anhui Acad Agr Sci, Rice Res Inst, Hefei 230031, Anhui, Peoples R China
关键词： Cell wall polysaccharides; glycosylation; Golgi apparatus; manganese; rice; transporter
期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）
ISSN： 0022-0957
年卷期： 2023 年 74 卷 6 期
页码：
收录情况： SCI
摘要： Manganese (Mn) is involved in many biochemical pathways as an enzyme cofactor, and is essential for maintaining metabolic processes in various plant cell compartments. Here, we determined the function of a rice (Oryza sativa) Mn transporter, PHOTOSYNTHESIS-AFFECTED MUTANT 71-LIKE 3 (OsPML3), belonging to the UNCHARACTERIZED PROTEIN FAMILY 0016 (UPF0016), in regulating Mn homeostasis and late-stage Golgi N-glycosylation. OsPML3 was highly expressed in rapidly developing tissues such as young leaves, root caps, lateral root primordia, and young anthers. Heterologous expression of OsPML3 restored the growth of Mn uptake-defective yeast strain Delta smf1 under Mn-limited conditions. Sub-cellular localization analysis revealed that OsPML3 localizes in the Golgi apparatus. At the vegetative stage, we observed necrotic root tips and lateral root primordia, and chlorotic young leaves in OsPML3 knockout lines under Mn-deficient conditions. Knocking out OsPML3 reduced the Mn content in the young leaves but did not affect the older leaves. Additionally, knocking out OsPML3 reduced the deposition of cell wall polysaccharides and the content of Le(a) (Lewis A structure)-containing N-glycan in roots and young leaves. OsPML3 knockout lines grown in the paddy field had reduced pollen fertility. Moreover, we found that the Lewis A structure was reduced in young anthers of OsPML3 knockout lines. Collectively, our results indicate that OsPML3 maintains Mn homeostasis in the Golgi apparatus of the rapidly developing rice tissues, and regulates the deposition of cell wall polysaccharides and late-stage Golgi N-glycosylation, especially biosynthesis of the Lewis A structure. OsPML3 regulates Mn homeostasis in the Golgi apparatus and plays an important role in the deposition of cell wall polysaccharides and late-stage Golgi N-glycosylation.
- 相关文献
作者其他论文 更多>>
-
Sub-Chronic Methomyl Exposure Induces Oxidative Stress and Inflammatory Responses in Zebrafish with Higher Female Susceptibility
作者：Li, Mingxiao;Chen, Xi;Song, Chao;Fan, Limin;Meng, Shunlong;Chen, Xi;Song, Chao;Fan, Limin;Qiu, Liping;Li, Dandan;Xu, Huimin;Meng, Shunlong;Xu, Jing;Mu, Xiyan;Xia, Bin;Ling, Jun
关键词：methomyl; oxidative stress; inflammation; sex differences; zebrafish
-
Identification of Copy Number Variations and Selection Signatures in Wannan Spotted Pigs by Whole Genome Sequencing Data: A Preliminary Study
作者：Zhang, Wei;Zhou, Mei;Liu, Linqing;Li, Xiaoyu;Su, Shiguang;Wang, Chonglong;Jiang, Yao;Ni, Zelan
关键词：Wannan spotted pig; Asian wild boar; copy number variation; selection signatures; whole genome resequencing
-
Sub-chronically exposing zebrafish to environmental levels of methomyl induces dysbiosis and dysfunction of the gut microbiota
作者：Li, Mingxiao;Chen, Xi;Song, Chao;Fan, Limin;Meng, Shunlong;Chen, Xi;Song, Chao;Fan, Limin;Qiu, Liping;Li, Dandan;Xu, Huimin;Meng, Shunlong;Mu, Xiyan;Xia, Bin;Ling, Jun
关键词：Methomyl; 16S rRNA; Metabolism; Inflammation; Apoptosis; Danio rerio
-
Copy number variants selected during pig domestication inferred from whole genome resequencing
作者：Zhang, Wei;Xu, Chengliang;Zhou, Mei;Liu, Linqing;Su, Shiguang;Wang, Chonglong;Ni, Zelan
关键词：Wanbei pig; Asian wild boar; copy number variation (CNV); selection signature; whole genome resequencing
-
Identification of Increased Grain Length 1 (IGL1), a novel gene encoded by a major QTL for modulating grain length in rice
作者：Niu, Jiayu;Wang, Fei;Yang, Chengcheng;Ye, Qiwen;Huang, Jingxian;La, Yumei;Wang, Qianqian;Hu, Tiange;Sang, Liran;Chen, Huhui;La, Honggui;Dai, Jie;Zhang, Peijiang;Zou, Yu;Zhai, Zhaoyu;Jin, Jian;Abdulmajid, Dina;Guo, Jingjing
关键词：
-
SC-Net: A new strip convolutional network model for rice seedling and weed segmentation in paddy field
作者：Liao, Juan;Chen, Minhui;Zhang, Kai;Xiong, Wei;Zhang, Shun;Kuang, Fuming;Zhu, Dequan;Zhou, Huiyu;Zou, Yu
关键词：Rice seedling and weed segmentation; Strip convolutional network model; PMCB; SMCB; AFF
-
Genome-Wide Detection for Runs of Homozygosity in Baoshan Pigs Using Whole Genome Resequencing
作者：Li, Wenjun;Wu, Lingxiang;Meng, Xintong;Huo, Jinlong;Fu, Guowen;Zhao, Guiying;Wu, Xudong;Zhang, Wei;Xiang, Decai;Yin, Zongjun
关键词：local breed; whole genome resequencing; runs of homozygosity; inbreeding coefficient; candidate gene
