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The Golgi-localized transporter OsPML3 is involved in manganese homeostasis and complex N-glycan synthesis in rice

文献类型： 外文期刊

作者： Xu, Ending 1 ; Wu, Mengyao 1 ; Liu, Yuanyuan 1 ; Tai, Yingping 1 ; Zha, Wenjuan 1 ; Gong, Changyi 1 ; Zou, Yu 2 ; Zhang, Peijiang 2 ; Zhang, Wei 1 ; Chen, Xi 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Dept Biochem & Mol Biol, Nanjing 210095, Jiangsu, Peoples R China

2.Anhui Acad Agr Sci, Rice Res Inst, Hefei 230031, Anhui, Peoples R China

关键词： Cell wall polysaccharides; glycosylation; Golgi apparatus; manganese; rice; transporter

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2023 年 74 卷 6 期

页码：

收录情况： SCI

摘要： Manganese (Mn) is involved in many biochemical pathways as an enzyme cofactor, and is essential for maintaining metabolic processes in various plant cell compartments. Here, we determined the function of a rice (Oryza sativa) Mn transporter, PHOTOSYNTHESIS-AFFECTED MUTANT 71-LIKE 3 (OsPML3), belonging to the UNCHARACTERIZED PROTEIN FAMILY 0016 (UPF0016), in regulating Mn homeostasis and late-stage Golgi N-glycosylation. OsPML3 was highly expressed in rapidly developing tissues such as young leaves, root caps, lateral root primordia, and young anthers. Heterologous expression of OsPML3 restored the growth of Mn uptake-defective yeast strain Delta smf1 under Mn-limited conditions. Sub-cellular localization analysis revealed that OsPML3 localizes in the Golgi apparatus. At the vegetative stage, we observed necrotic root tips and lateral root primordia, and chlorotic young leaves in OsPML3 knockout lines under Mn-deficient conditions. Knocking out OsPML3 reduced the Mn content in the young leaves but did not affect the older leaves. Additionally, knocking out OsPML3 reduced the deposition of cell wall polysaccharides and the content of Le(a) (Lewis A structure)-containing N-glycan in roots and young leaves. OsPML3 knockout lines grown in the paddy field had reduced pollen fertility. Moreover, we found that the Lewis A structure was reduced in young anthers of OsPML3 knockout lines. Collectively, our results indicate that OsPML3 maintains Mn homeostasis in the Golgi apparatus of the rapidly developing rice tissues, and regulates the deposition of cell wall polysaccharides and late-stage Golgi N-glycosylation, especially biosynthesis of the Lewis A structure. OsPML3 regulates Mn homeostasis in the Golgi apparatus and plays an important role in the deposition of cell wall polysaccharides and late-stage Golgi N-glycosylation.

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