Evaluation of Recombinase Polymerase Amplification Assay for Detecting Meloidogyne javanica
文献类型： 外文期刊
作者： Chi, Yuan-Kai 1 ; Zhao, Wei 1 ; Ye, Meng-di 2 ; Ali, Farman 3 ; Wang, Tao 1 ; Qi, Ren-de 1 ;
作者机构： 1.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei, Peoples R China
2.Anhui Agr Univ, Sch Plant Protect, Hefei, Peoples R China
3.Abdul Wali Khan Univ, Dept Agr, Mardan, Pakistan
关键词： detection; lateral flow dipstick; Meloidogyne javanica; recombinase polymerase amplification
期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）
ISSN： 0191-2917
年卷期： 2020 年 104 卷 3 期
页码：
收录情况： SCI
摘要： Meloidogyne javanica is one of the most widespread and economically important nematodes in many countries, including China. In this study, a recombinase polymerase amplification (RPA) assay was evaluated for the detection of M. javanica based on the sequences of a sequence-characterized amplified regions marker gene segment. The RPA assay specifically detected M. javanica from individual juvenile or adult female, M. javanica-induced galls, and nematodes in the soil samples. The detection limit of M. javanica RPA assay was 1 pg of purified genomic DNA, 0.01 adult female, or 0.1 second-stage juvenile, which was 10 times more sensitive than conventional PCR assay. Furthermore, combined with lateral flow dipstick (LFD), a visual detection method of LFD-RPA assay was developed, which is suitable for onsite surveys and routine diagnostics. Results indicate that the RPA assay is rapid, sensitive, and reliable for detection and molecular identification of M. javanica.
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