您好，欢迎访问安徽省农业科学院 机构知识库！

Identification, expression and miRNA targeting of auxin response factor genes related to phyllody in the witches' broom disease of jujube

文献类型： 外文期刊

作者： Ma, Fuli 1 ; Huang, Jinqiu 1 ; Yang, Jian 1 ; Zhou, Junyong 1 ; Sun, Qibao 2 ; Sun, Jun 1 ;

作者机构： 1.Anhui Agr Univ, Coll Hort, 130 West Changjiang Rd, Hefei 230006, Anhui, Peoples R China

2.Anhui Acad Agr Sci, Hort Res Inst, 40 South Nongke Rd, Hefei 230031, Anhui, Peoples R China

3.Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, 130 West Changjiang Rd, Hefei 230006, Anhui, Peoples R China

关键词： Ziziphus jujuba; Phytoplasma; Jujube witches' broom; Auxin response; Floral development

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2020 年 746 卷

页码：

收录情况： SCI

摘要： Jujube witches' broom (JWB) disease, associated with the presence of phytoplasmas, induces huge crop losses in the woody perennial fruit tree Ziziphus jujuba. An imbalance in the phytohormone auxin is thought to be a key factor in the development of the witches' broom symptoms, and in the alteration of floral development into leafy structures, termed phyllody. The Auxin Response Factor (ARF) gene family controls auxin-responsive gene expression during plant growth and development. However, it remains unknown if the ARF genes are involved in the formation of leaf-like flowers. In the present study, sixteen jujube ARF genes were identified bioinformatically and annotated based on the Z. jujuba cv. Dongzao genome. The ZjARFs were homologous to 12 out of the 23 Arabidopsis ARFs and were distributed in 8 jujube chromosomes and 3 unmapped scaffolds. Phylogenetic analysis grouped the ZjARFs into three classes. Spatio-temporal expression analysis revealed that the ZjARF genes were differentially expressed among different tissues during normal development. The expression of seven ZjARF genes was significantly decreased from flower buds to flowering. JWB-infected jujube plants developed the typical phyllody symptoms and showed lower auxin accumulation during floral development. ZjARF1, ZjARF2, ZjARF3, ZjARF4 and ZjARF8 resulted differentially regulated after phytoplasma infection. ZjARF4 was downregulated before and during floral development in phytoplasma-infected plants, but it was significantly upregulated before flowering and down-regulated during flowering in the healthy plants. Target site analysis showed that miRNA167, miRNA529 and miRNA2950 could directly target ZjARF4. Together, the data showed that the auxin-controlled ARF4 gene is likely involved in the disruption of floral development in phytoplasma-infected jujube plants.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台