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The effector SJP3 interferes with pistil development by sustaining SHORT VEGETATIVE PHASE 3 expression in jujube

文献类型： 外文期刊

作者： Deng, Mingsheng 1 ; Ma, Fuli 1 ; Zhai, Liping 1 ; Zhang, Xinyue 1 ; Zhang, Ning 1 ; Zheng, Yunyan 1 ; Chen, Wei 1 ; Zhou, Wenmin 1 ; Pang, Kaixue 1 ; Zhou, Junyong 1 ; Sun, Qibao 2 ; Sun, Jun 1 ;

作者机构： 1.Anhui Agr Univ, Coll Hort, Anhui Prov Key Lab Hort Crop Qual Biol, 130 West Changjiang Rd, Hefei 230036, Anhui, Peoples R China

2.Anhui Acad Agr Sci, Hort Res Inst, 40 South Nongke Rd, Hefei 230001, Anhui, Peoples R China

期刊名称：PLANT PHYSIOLOGY （ 影响因子：6.9； 五年影响因子：7.7 ）

ISSN： 0032-0889

年卷期： 2024 年 196 卷 3 期

页码：

收录情况： SCI

摘要： Jujube witches' broom (JWB) is a phytoplasma disease that causes severe damage to jujube (Ziziphus jujuba) crops worldwide. Diseased jujube plants show enhanced vegetative growth after floral reversion, including leafy flower structures (phyllody) and the fourth whorl converting into a vegetative shoot. In previous research, secreted JWB protein 3 (SJP3) was identified as an inducer of phyllody. However, the molecular mechanisms of SJP3-mediated pistil reversion remain unknown. Here, the effector SJP3 was found to interact with the MADS-box protein SHORT VEGETATIVE PHASE 3 (ZjSVP3). ZjSVP3 was expressed in young leaves and during the initial flower bud differentiation of healthy jujube-bearing shoots but was constitutively expressed in JWB phytoplasma-infected flowers until the later stage of floral development. The SJP3 effector showed the same expression pattern in the diseased buds and promoted ZjSVP3 accumulation in SJP3 transgenic jujube calli. The N-terminal domains of ZjSVP3 contributed to its escape from protein degradation in the presence of SJP3. Heterologous expression of ZjSVP3 in Nicotiana benthamiana produced typical pistil abnormalities, including trichome-enriched style and stemlike structures within the leaflike ovary, which were consistent with those in the mildly malformed lines overexpressing SJP3. Furthermore, ectopic expression of ZjSVP3 directly bound to the zinc finger protein 8 (ZjZFP8) and MADS-box gene SHATTERPROOF 1 (ZjSHP1) promoters to regulate their expression, resulting in abnormal pistil development. Overall, effector SJP3-mediated derepression of ZjSVP3 sustained its expression to interfere with pistil development, providing insight into the mechanisms of pistil reversion caused by JWB phytoplasma in specific perennial woody plant species. A phytoplasma effector stabilizes the ectopic expression of a flowering repressor protein, resulting in the misexpression of related genes and the reversal of pistils into vegetative structures.

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