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Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14

文献类型： 外文期刊

作者： Yu, Qing 1 ; Li, Jiyu 2 ; Qin, Gaihua 2 ; Liu, Chunyan 2 ; Cao, Zhen 2 ; Jia, Botao 2 ; Xu, Yiliu 2 ; Li, Guixiang 1 ; Yang, Yuan 2 ; Su, Ying 1 ; Zhang, Huping 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China

2.Anhui Acad Agr Sci, Inst Hort Res, Key Lab Hort Crop Genet Improvement & Ecophysiol, Key Lab Fruit Qual & Dev Biol, Hefei 230031, Peoples R China

关键词： pomegranate; seed hardness; inner seed coat; ABCG transporter; evolution; lignin biosynthesis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 19 期

页码：

收录情况： SCI

摘要： ATP-binding cassette subfamily G (ABCG) proteins play important roles in plant growth and development by transporting metabolites across cell membranes. To date, the genetic characteristics and potential functions of pomegranate ABCG proteins (PgrABCGs) have remained largely unknown. In this study, we found that 47 PgrABCGs were divided into five groups according to a phylogenetic analysis; groups I, II, III, and IV members are half-size proteins, and group V members are full-size proteins. PgrABCG14, PgrABCG21, and PgrABCG47 were highly expressed in the inner seed coat but had very low expression levels in the outer seed coat, and the expression levels of these three PgrABCG genes in the inner seed coats of hard-seeded pomegranate 'Dabenzi' were higher than those of soft-seeded pomegranate 'Tunisia'. In addition, the expression of these three PgrABCG genes was highly correlated with the expression of genes involved in lignin biosynthesis and hormone signaling pathways. The evolution of PgrABCG14 presents a highly similar trend to the origin and evolution of lignin biosynthesis during land plant evolution. Ectopic expression of PgrABCG14 in Arabidopsis promoted plant growth and lignin accumulation compared to wild type plants; meanwhile, the expression levels of lignin biosynthesis-related genes (CAD5, C4H, and Prx71) and cytokinin response marker genes (ARR5 and ARR15) were significantly upregulated in transgenic plants, which suggests the potential role of PgrABCG14 in promoting plant growth and lignin accumulation. Taken together, these findings not only provide insight into the characteristics and evolution of PgrABCGs, but also shed a light on the potential functions of PgrABCGs in seed hardness development.

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