文献类型： 外文期刊

作者： Ren, Zhongying ¹ ; Yu, Daoqian ¹ ; Yang, Zhaoen ¹ ; Li, Changfeng ¹ ; Qanmber, Ghulam ¹ ; Li, Yi ¹ ; Li, Jie ¹ ; Liu, Zhao ¹ ; Lu, Lili ¹ ; Wang, Lingling ¹ ; Zhang, Hua ¹ ; Chen, Quanjia ¹ ; Li, Fuguang ² ; Yang, Zuoren ³ ;

作者机构： 1.Xinjiang Research Base, State Key Laboratory of Cotton Biology, Xinjiang Agriculture University, Urumqi, China;Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China

2.Chinese Acad Agr Sci, Inst Cotton Res, Anyang, Peoples R China;^Xinjiang Agr Univ, State Key Lab Cotton Biol, Xinjiang Res Base, Urumqi, Peoples R China

3.;Xinjiang Agr Univ, State Key Lab Cotton Biol, Xinjiang Res Base, Urumqi, Peoples R China;^Chinese Acad Agr Sci, Inst Cotton Res, Anyang, Peoples R China

4.Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China;Cotton Research Institute, Anhui Academy of Agricultural Sciences, Hefei, China

5.Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China

6.Xinjiang Research Base, State Key Laboratory of Cotton Biology, Xinjiang Agriculture University, Urumqi, China

关键词： Gossypium hirsutum L;GhMIKCs;phylogeny;structure;expression patterns;flower

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Cotton is one of the major world oil crops. Cottonseed oil meets the increasing demand of fried food, ruminant feed, and renewable bio-fuels. MADS intervening keratin-like and C-terminal (MIKC)-type MADS-box genes encode transcription factors that have crucial roles in various plant developmental processes. Nevertheless, this gene family has not been characterized, nor its functions investigated, in cotton. Here, we performed a comprehensive analysis of MIKC-type MADS genes in the tetraploid Gossypium hirsuturn L., which is the most widely cultivated cotton species. In total, 110 GhMIKC genes were identified and phylogenetically classified into 13 subfamilies. The Flowering locus C (FLC) subfamily was absent in the Gossypium hirsutum L. genome but is found in Arabidopsis and Vitis vinifera L. Among the genes, 108 were distributed across the 13 A and 12 of the D genome's chromosomes, while two were located in scaffolds. GhMIKCs within subfamilies displayed similar exon/intron characteristics and conserved motif compositions. According to RNA-sequencing, most MIKC genes exhibited high flowering-associated expression profiles. A quantitative real-time PCR analysis revealed that some crucial MIKC genes determined the identities of the five flower organs. Furthermore, the overexpression of GhAGL17.9 in Arabidopsis caused an early flowering phenotype. Meanwhile, the expression levels of the flowering-related genes CONSTANS (CO), LEAFY (LFY) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) were significantly increased in these lines. These results provide useful information for future studies of GhMIKCs' regulation of cotton flowering.