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An RpoN-dependent PEP-CTERM gene is involved in floc formation of an Aquincola tertiaricarbonis strain

文献类型： 外文期刊

作者： Xia, Ming 1 ; Yu, Dianzhen 3 ; Chen, Han 3 ; Dai, Jingcheng 5 ; Gao, Na 6 ; Li, Shuyang 5 ; Bi, Xuezhi 7 ; Qiu, Dongru 5 ;

作者机构： 1.Jianghan Univ, Hubei Engn Res Ctr Protect & Utilizat Special Biol, Sch Life Sci, Wuhan 430056, Peoples R China

2.Jianghan Univ, Hubei Key Lab Environm & Hlth Effects Persistent T, Wuhan 430056, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

4.Chinese Acad Sci, Inst Nutr Sci, SIBS, Shanghai 200031, Peoples R China

5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China

6.Anhui Acad Agr Sci, Fisheries Res Inst, Hefei 230031, Peoples R China

7.Agcy Sci Technol & Res, Bioproc Technol Inst, Singapore 138668, Singapore

关键词： Bacterial floc formation; PEP-CTERM proteins; Flocculation; RpoN sigma factor; Aquincola tertiaricarbonis

期刊名称：BMC MICROBIOLOGY （ 影响因子：4.2； 五年影响因子：4.7 ）

ISSN： 1471-2180

年卷期： 2023 年 23 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundThe floc is a characteristic of microbial aggregate growth, displaying cloudy suspensions in water. Floc formation has been demonstrated in a series of bacteria and the floc-forming bacteria play a crucial role in activated sludge (AS) process widely used for municipal sewage and industrial wastewater treatment over a century. It has been demonstrated that some exopolysaccharide biosynthesis genes and the sigma factor (sigma54 or rpoN) were required for floc forming in some bacteria. However, the mechanism underlying the floc formation stills need to be elucidated.ResultsIn this study, we demonstrate that a TPR (Tetratricopeptide repeats) protein-encoding gene prsT is required for floc formation of Aquincola tertiaricarbonis RN12 and an upstream PEP-CTERM gene (designated pepA), regulated by RpoN1, is involved in its floc formation but not swarming motility and biofilm formation. Overexpression of PepA could rescue the floc-forming phenotype of the rpoN1 mutant by decreasing the released soluble exopolysaccharides and increasing the bound polymers.ConclusionOur results indicate that the wide-spread PEP-CTERM proteins play an important role in the self-flocculation of bacterial cells and may be a component of extracellular polymeric substances required for floc-formation.

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