Cloning and expression analysis of two pro-inflammatory cytokines, IL-1 beta and its receptor, IL-1R2, in the Asian swamp eel Monopterus albus
文献类型： 外文期刊
作者： Xu, Q. Q. 1 ; Xu, P. 2 ; Zhou, J. W. 2 ; Pan, T. S. 3 ; Tuo, R. 2 ; Ai, K. 2 ; Yang, D. Q. 1 ;
作者机构： 1.Yangtze Univ, Engn Res Ctr Ecol & Agr, Use Wetland, Minist Educ, Jingzhou 434020, Hubei Province, Peoples R China
2.Yangtze Univ, Sch Anim Sci, Jingzhou 434020, Hubei Province, Peoples R China
3.Anhui Acad Agr Sci, Inst Fisheries, Hefei 230031, Anhui, Peoples R China
4.Hubei Collaborat Innovat Ctr Freshwater Aquacultu, Wuhan 430070, Peoples R China
关键词： interleukin-1 beta;IL-1 beta;IL1 receptor 2;gene structure;inducible expression;Asian swamp eel;Monopterus albus
期刊名称：MOLECULAR BIOLOGY （ 影响因子：1.374； 五年影响因子：1.266 ）
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收录情况： SCI
摘要： Interleukin-1 beta (IL-1 beta) is the prototypic pro-inflammatory cytokine, whose functions are mediated through interaction with its receptors (IL-1R1 and IL-1R2). Herein, we cloned the full-length cDNA and genomic DNA of IL-1 beta and IL-1R2 in the Asian swamp eel (Monopterus albus). The eel IL-1 beta cDNA encodes a putative polypeptide of 246 amino acids. The protein sequence includes a typical IL-1 family signature, but lacked an interleukin-converting enzyme cleavage site. The genomic DNA of eel IL-1 beta was 2520 bp and comprised five exons and four introns. The eel IL-1R2 cDNA encoded a putative propeptide of 423 amino acid residues, comprising a signal peptide, a transmembrane region and two Ig-like domains in the extracellular region. Similar to other vertebrates, the genomic DNA of the eel IL-1R2 has nine exons and eight introns. Real-time PCR analysis indicated that IL-1 beta and IL-1R2 were constitutively expressed in all tissues, especially in the liver and immune-related organs. After infection with Aeromonas hydrophila, the transcript levels of IL-1 beta and IL-1R2 were induced in the head kidney and spleen, reaching their highest levels at 6 h post injection. In vitro, IL-1 beta and IL-1R2 mRNA levels were also upregulated rapidly at 1h post infection with A. hydrophila. Furthermore, acanthocephalan Pallisentis (Neosentis) celatus could induce the expression of both genes in the head kidney and intestine. In infected intestines, the transcript levels of IL-1 beta and IL-1R2 were increased by 21.4-fold and 20.8-fold, respectively, relative to the control. The present study indicated that IL-1 beta and IL-1R2 play an important role in inflammation and host defense, especially in the antiacanthocephalan response.
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