文献类型： 外文期刊

作者： Fang, Qing ¹ ; Wang, Jing-Fei ¹ ; Zha, Xue-Qiang ¹ ; Cui, Shao-Hua ¹ ; Cao, Lei ² ; Luo, Jian-Ping ¹ ;

作者机构： 1.Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China

2.Anhui Acad Agr Sci, Inst Agroprod Proc, Chongqing, Peoples R China

关键词： Laminaria japonica;Polysaccharides;Immunostimulation;Macrophage;TLR4

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

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收录情况： SCI

摘要： In this work, a novel water-soluble homogeneous polysaccharide (LJP-31) with a molecular mass of 2.24 x 10(6) Da was isolated and purified from Laminaria japonica using DEAE-cellulose and Sephacryl S500 chromatography. Results showed that LJP-31 mainly consists of arabinose, mannose, glucose and galactose in a molar ratio of 1.0:7.8:6.6:0.8. LJP-31 exhibited significant stimulation on macrophages and enhanced the production of NO, TNF-alpha, IL-1 beta, IL-6 and IL-10 as well as the up-regulation of their gene expressions. Western blot analysis suggested that LJP-31 has the positive effects on the translocation of NF-kappa B p65 from cytoplasm to nucleus and the phosphorylation of I kappa B alpha, ERK1/2, JNK1/2 and P38 in macrophages. Flow cytometric and confocal laser-scanning microscopy analysis indicated that toll-like receptor 4 (TLR4) was at least one of the recognition receptors of LJP-31 on the plasma membrane of macrophages. Taken together, LJP-31 may exert its immunostimulating potency via TLR4 activation of MAPK and NF-kappa B signaling pathways. (C) 2015 Elsevier Ltd. All rights reserved.