您好，欢迎访问安徽省农业科学院 机构知识库！

Proteomics and pathway analysis of N-glycosylated mammary gland proteins in response to Escherichia coli mastitis in cattle

文献类型： 外文期刊

作者： Yang, Yongxin 1 ; Shen, Weijun 2 ; Zhao, Xiaowei 1 ; Zhao, Huiling 1 ; Huang, Dongwei 1 ; Cheng, Guanglong 1 ;

作者机构： 1.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Hefei 230031, Peoples R China

2.Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China

关键词： Dairy cow;Mastitis;Escherichia coli;Glycosylation;Proteome

期刊名称：VETERINARY JOURNAL （ 影响因子：2.688； 五年影响因子：2.857 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The aim of this study was to investigate the N-linked glycosylated protein profile of mammary tissue from healthy cows and cows with mastitis due to Escherichia coli, in order to understand the molecular mechanisms of the host response to mastitis. N-glycopeptides were enriched with a lectin mixture and identified through high-accuracy mass spectrometry. A total of 551 N-glycosylation sites, corresponding to 294 proteins, were identified in the mammary tissues of healthy cows; these glycoproteins were categorised into three functional groups and clustered into 11 specific pathways. A total of 511 N-glycosylation sites, corresponding to 283 glycosylated proteins, were detected in the mammary tissues of cows with E. coli mastitis. There were differences in N-glycosylation sites in 98 proteins in the mammary tissues of healthy cows and cows with mastitis due to E. coli. Most proteins with altered glycosylation were those involved in responses to stress, cell adhesion and the immune response, and were assigned to five specific pathways based on their gene ontology annotation. The results from this study show that the glycosylated protein profile in the mammary tissues of healthy and mastitic cows are different, and altered glycoproteins are associated with several pathways, including the lysosome and O-glycan biosynthesis pathways

  • 相关文献

[1]Comparative proteomics analysis of plasma proteins during the transition period in dairy cows with or without subclinical mastitis after calving. Yang, Y. X.,Wang, J. Q.,Bu, D. P.,Li, S. S.,Yuan, T. J.,Zhou, L. Y.,Yang, J. H.,Sun, P.,Yang, Y. X..

[2]N-glycosylation proteomic characterization and cross-species comparison of milk whey proteins from dairy animals. Yang, Yongxin,Zheng, Nan,Zhao, Xiaowei,Zhang, Yangdong,Han, Rongwei,Zhao, Shengguo,Yang, Jinhui,Li, Songli,Guo, Tongjun,Zang, Changjiang,Wang, Jiaqi,Yang, Yongxin,Zhao, Xiaowei,Han, Rongwei.

[3]N-glycosylation proteomic characterization and cross-species comparison of milk fat globule membrane proteins from mammals. Yang, Yongxin,Zheng, Nan,Zhao, Xiaowei,Zhang, Yangdong,Han, Rongwei,Ma, Lu,Zhao, Shengguo,Li, Songli,Guo, Tongjun,Zang, Changjiang,Wang, Jiaqi,Yang, Yongxin,Zhao, Xiaowei,Wang, Weiyu.

[4]Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coil mastitis. Zhao, Xiao-wei,Yang, Yong-xin,Huang, Dong-wei,Cheng, Guang-long,Zhao, Hui-ling.

[5]Effects of duodenal infusion of free alpha-linolenic acid on the plasma and milk proteome of lactating dairy cows. Yang, Y. X.,Wang, J. Q.,Yuan, T. J.,Bu, D. P.,Yang, J. H.,Sun, P.,Zhou, L. Y.,Yang, Y. X.. 2013

[6]Comparison of base substitutions in response to nitrogen ion implantation and Co-60-gamma ray irradiation in Escherichia coli. Xie, CX,Xu, A,Wu, LJ,Yao, JM,Yang, JB,Yu, ZL. 2004

[7]Comparative Proteomic Analysis of Plasma from Clinical Healthy Cows and Mastitic Cows. Yang Yong-xin,Zhao Xing-xu,Zhang Yong,Yang Yong-xin. 2009

[8]Determination of changes in bovine plasma and milk proteins during naturally occurring Escherichia coli mastitis by comparative proteomic analysis. Yang, Yongxin,Zhao, Xiaowei,Huang, Dongwei,Zhao, Huiling,Cheng, Guanglong,Cao, Suizhong,Cao, Suizhong.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台