DNA methylation alterations of upland cotton (Gossypium hirsutum) in response to cold stress
文献类型： 外文期刊
作者： Fan, Hong Hong 1 ; Wei, Jun 1 ; Li, Ting Chun 1 ; Li, Zheng Peng 1 ; Guo, Ning 1 ; Cai, Yong Ping 1 ; Lin, Yi 1 ;
作者机构： 1.Anhui Agr Univ, Dept Life Sci, Hefei 230036, Peoples R China
2.Anhui Acad Agr Sci, Tobacco Res Inst, Hefei 230031, Peoples R China
关键词： Cold stress;MSAP;DNA methylation;Gene expression
期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）
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收录情况： SCI
摘要： DNA methylation plays an important role in regulating gene expression in plants. In the experiment, we studied effects of cold on DNA methylation variation in upland cotton. Using the methylation-sensitive amplified polymorphism procedure, we chose 66pairs of selective amplification primers to assess the status and levels of cytosine methylation. The hemimethylation of the external cytosine and the full methylation of the internal cytosine were scored. As a result, cold triggered the demethylation of hemimethylated or internally full methylated cytosine. With the prolongation of cold treatment, the demethylation loci increased and the methylation loci decreased. Nevertheless, this change could be reverted when cotton was subsequently recovered under normal temperature. In addition, 29 polymorphic bands that appeared in the electrophoretogram were sequenced. By homologous alignment analysis, most of these 29 fragments were identified as genes or DNA clones involved in abiotic stress response. The variation in methylation loci existed at both coding and non-coding regions. Furthermore, the expression of the abiotic stress-related genes, GhCLSD (Seq21), GhARK (Seq22), GhARM (Seq15, Seq18, Seq19 and Seq21) and GhTPS (Seq8), were tested. The results revealed that cold treatment induced down-regulation of GhCLSD, GhARK and GhARM, but up-regulated the expression of GhTPS. These changes were in accordance with the alteration of DNA methylation. Thus, cold may affect the gene expression via changing themethylation status in the cytosine nucleotide.
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