文献类型： 外文期刊

作者： Sun, JS ¹ ; Chen, FS ² ; Xu, WH ² ;

作者机构： 1.Univ Sci & Technol China, Sch Life Sci, Dept Mol & Cell Biol, Hefei 230027, Peoples R China

2.Univ Sci & Technol China, Sch Life Sci, Dept Mol & Cell Biol, Hefei 230027, Peoples R China; Anhui Acad Agr Sci, Inst Sericultural Res, Hefei 230036, Peoples R China

关键词： diapause hormone;in situ hybridization;immunocytochemistry;embryo;larva;Bombyx mori

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： In this paper, the distribution and expression of diapause hormone (DH) at mRNA and protein levels in the central nervous system of Bombyx mori embryo and larva were studied using whole-mount in situ hybridization and immunocytochemistry. Whole-mount immunocytochemistry revealed that the distribution of the DH-like immunoreactivity was throughout the central nervous system including the brain, suboesophageal ganglion (SG) and thoracic ganglia (TG); and that the corpus cardiacum and terminal abdominal ganglion may be the site for DH release due to the presence of strong immunoreactivity. In situ hybridization with the probe labeled by digoxigenin shows that the Bom-DH mRNA was also localized in the mandibular, maxillary, labial cell clusters. In addition, a pair of lateral neurons in the SG and a pair of ventral midline neurons in each TG expressing the Bom-DH transcript were also identified. These results were consistent with the localization of Bom-DH mRNA in larva by in situ hybridization and the distribution of the gene by RT-PCR, which is some different from the results reported previously.