Genome-Wide Transcriptome Analysis of a Virulent sRNA, Trans217, in Xanthomonas oryzae pv. oryzae (Xoo), the Causative Agent of Rice Bacterial Blight
文献类型： 外文期刊
作者： Hu, Yiqun 1 ; Zhang, Jianjian 3 ; Zhang, Aifang 1 ;
作者机构： 1.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei 230031, Peoples R China
2.Anhui Prov Key Lab Pesticide Resistance Management, Hefei 230031, Peoples R China
3.Univ Sci & Technol China, Dept Sci Res, Hefei 230026, Peoples R China
关键词： Xanthomonas oryzae pv. oryzae; sRNA trans217; transcriptome; gene differential expression; oxidative stress
期刊名称：MICROORGANISMS （ 影响因子：4.2； 五年影响因子：4.6 ）
ISSN：
年卷期： 2024 年 12 卷 8 期
页码：
收录情况： SCI
摘要： Small non-coding RNAs (sRNAs) act as post-transcriptional regulators to participate in many cellular processes. Among these, sRNA trans217 has been identified as a key virulent factor associated with pathogenicity in rice, triggering hypersensitive reactions in non-host tobacco and facilitating the secretion of the PthXo1 effector in Xanthomonas oryzae pv. oryzae (Xoo) strain PXO99(A). Elucidating potential targets and downstream regulatory genes is crucial for understanding cellular networks governing pathogenicity and plant resistance. To explore the targets regulated by sRNA trans217, transcriptome sequencing was carried out to assess differential expression genes (DEGs) between the wild-type strain PXO99(A) and a mutant lacking the sRNA fragment under both virulence-inducing or normal growth conditions. DEG analysis revealed that sRNA trans217 was responsible for diverse functions, such as type III secretion system (T3SS), glutamate synthase activity, and oxidative stress response. Three genes were selected for further investigation due to their significant differential expression and biological relevance. Deletion of PXO_RS08490 attenuated the pathogenicity of Xoo in rice and reduced the tolerance level of PXO99(A) to hydrogen peroxide. These findings suggest a regulatory role of sRNA trans217 in modulating bacterial virulence through multiple gene targets, either directly or indirectly.
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