Identifying candidate genes involved in trichome formation on carrot stems by transcriptome profiling and resequencing
文献类型： 外文期刊
作者： Wu, Zhe 1 ; Yang, Xuan 1 ; Zhao, Yu-xuan 1 ; Jia, Li 2 ;
作者机构： 1.Shanxi Agr Univ, Coll Hort, Taigu 030801, Peoples R China
2.Anhui Acad Agr Sci, Inst Hort, Key Lab Genet Improvement & Ecophysiol Hort Crop A, Hefei 230001, Peoples R China
关键词： carrot; trichome; resequencing; transcriptome; candidate genes
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.8； 五年影响因子：4.8 ）
ISSN： 2095-3119
年卷期： 2022 年 21 卷 12 期
页码：
收录情况： SCI
摘要： Trichomes are specialized structures developed from epidermal cells and can protect plants against biotic and abiotic stresses. Trichomes cover carrots during the generative phase. However, the morphology of the carrot trichomes and candidate genes controlling the formation of trichomes are still unclear. This study found that carrot trichomes were non -glandular and unbranched hairs distributed on the stem, leaf, petiole, pedicel, and seed of carrot. Resequencing analysis of a trichome mutant with sparse and short trichomes (sst) and a wild type (wt) with long and dense trichomes on carrot stems was conducted. A total of 15 396 genes containing nonsynonymous mutations in sst were obtained, including 42 trichome-related genes. We also analyzed the transcriptome of the trichomes on secondary branches when these secondary branches were 10 cm long between wt and sst and obtained 6 576 differentially expressed genes (DEGs), including 24 trichome-related genes. qRT-PCR validation exhibited three significantly up-regulated DEGs, 20 significantly down -regulated, and one with no difference. We considered both the resequencing and transcriptome sequencing analyses and found that 12 trichome-related genes that were grouped into five transcription factor families containing nonsynonymous mutations and significantly down-regulated in sst. Therefore, these genes are potentially promising candidate genes whose nonsynonymous mutations and down-regulation may result in scarce and short trichomes mutation on carrot stems in sst.
- 相关文献
作者其他论文 更多>>
-
The Physiological Mechanism of Exogenous Melatonin Regulating Salt Tolerance in Eggplant Seedlings
作者：Zhang, Yu;Yang, Dekun;Lu, Xiaomin;Zhang, Yu;Jia, Li;Wang, Han;Jiang, Haikun;Ding, Qiangqiang;Yang, Dekun;Yan, Congsheng;Jia, Li;Jiang, Haikun;Ding, Qiangqiang;Yan, Congsheng;Jia, Li;Jiang, Haikun;Ding, Qiangqiang;Yan, Congsheng
关键词：exogenous melatonin; eggplant seedlings; mitigation effect; secondary salinization
-
Fabrication of SA/CMC composite membrane embedded with ascorbic acid and cross-linked through propylene glycol: The study on the preservation effects on fresh-cut eggplants
作者：Wei, Lanlan;Yang, Yanyan;Zhu, Shuaijie;Jia, Li
关键词：preservation; membrane; food safety; encapsualtion; membrane processes
-
Transcriptomic and metabolomic analysis reveals the molecular mechanism of exogenous melatonin improves salt tolerance in eggplants
作者：Wang, Han;Jiang, Haikun;Ding, Qiangqiang;Wang, Yan;Wang, Mingxia;Yan, Congsheng;Jia, Li;Wang, Han;Zhang, Yu;Jiang, Haikun;Ding, Qiangqiang;Wang, Yan;Wang, Mingxia;Yan, Congsheng;Jia, Li;Wang, Han;Zhang, Yu;Jiang, Haikun;Ding, Qiangqiang;Wang, Yan;Wang, Mingxia;Yan, Congsheng;Jia, Li
关键词：melatonin; salt stress; eggplant; transcriptomic; metabolomic; alpha-linolenic acid
-
Transcriptomic and Metabolomic Analysis Reveals the Mechanism of H18 Pepper Color Change
作者：Wang, Han;Jia, Li;Yan, Congsheng;Ding, Qiangqiang;Wang, Yan;Hong, Xiujing;Song, Tingting;Jiang, Haikun;Wang, Han;Jia, Li;Yan, Congsheng;Ding, Qiangqiang;Wang, Yan;Hong, Xiujing;Song, Tingting;Jiang, Haikun;Wang, Han;Jia, Li;Yan, Congsheng;Ding, Qiangqiang;Wang, Yan;Hong, Xiujing;Song, Tingting;Jiang, Haikun;Li, Dongchen;Manzoor, Muhammad Aamir
关键词：chili pepper; pigments; transcriptomic; metabolomic; anthocyanin; carotenoid
-
Genome-Wide Identification and Expression Analysis of the Pepper β-1,3-gucanase Gene Family in Response to Phytophthora capsici Stresses
作者：Wang, Han;Wang, Yanping;Song, Tingting;Yan, Congsheng;Jia, Li;Jiang, Haikun;Wang, Han;Wang, Yanping;Song, Tingting;Yan, Congsheng;Jia, Li;Jiang, Haikun;Wang, Han;Wang, Yanping;Song, Tingting;Yan, Congsheng;Jia, Li;Jiang, Haikun;Li, Dongchen;Zhang, Yu
关键词：pepper; beta-1,3-glucanase;
Phytophthora capsici ; resistant -
The DIR Gene Family in Watermelon: Evolution, Stress Expression Profiles, and Functional Exploration of ClDIR8
作者：Zhang, Kaijing;Ding, Qiangqiang;Jia, Li;Yan, Congsheng;Zhang, Kaijing;Wang, Zhu;Tian, Huiyu;Gao, Jiong;Cui, Rongjing;Shu, Yingjie;Yan, Congsheng;Zhang, Kaijing;Ding, Qiangqiang;Jia, Li;Yan, Congsheng;Ding, Qiangqiang;Jia, Li;Yan, Congsheng
关键词：watermelon; DIR; gene family; bioinformatics; expression analysis; subcellular localization; interacting proteins
-
Genome-Wide Analysis of the NBS-LRR Gene Family and SSR Molecular Markers Development in Solanaceae
作者：Song, Xiaoming;Jia, Li;Song, Xiaoming;Jia, Li;Song, Xiaoming;Li, Chunjin;Liu, Zhuo;Shen, Shaoqin;Yu, Tong;Li, Nan;Zhou, Rong;Zhou, Rong
关键词：Solanaceae;
NBS-LRR ; phylogenetic analysis; whole genome duplication/triplication (WGD/T); SSR development
