文献类型： 外文期刊
作者： Xu, Rongfang 1 ; Kong, Fanna 1 ; Qin, Ruiying 1 ; Li, Juan 1 ; Liu, Xiaoshuang 1 ; Wei, Pengcheng 1 ;
作者机构： 1.Anhui Acad Agr Sci, Rice Res Inst, Key Lab Rice Genet Breeding Anhui Prov, Hefei 230031, Peoples R China
2.Anhui Agr Univ, Coll Agron, Hefei 230036, Peoples R China
关键词： base editing; CRISPR; dual-base editor; genome editing; rice
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）
ISSN： 1672-9072
年卷期： 2021 年 63 卷 9 期
页码：
收录情况： SCI
摘要： An enhanced CDA-like (eCDAL) was established from Japanese lamprey CDA1-like 4 to achieve a high editing frequency in a broad region as a C-terminal cytosine base editors (CT-CBE). Then, a novel plant dual-base editor version 1(pDuBE1) was developed by integrating TadA-8e into eCDAL. The editing efficiency of pDuBE1 could reach to 87.6%, with frequencies of concurrent A-to-G and C-to-T conversions as high as 49.7% in stably transformed plant cells. Our results showed that pDuBE1 could mediate robust dual editing in plant genome, providing a powerful manipulation tool for precise crop breeding and screening platforms for in planta direct evolution.
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