文献类型： 外文期刊
作者： Xu, Rongfang 1 ; Kong, Fanna 1 ; Qin, Ruiying 1 ; Li, Juan 1 ; Liu, Xiaoshuang 1 ; Wei, Pengcheng 1 ;
作者机构： 1.Anhui Acad Agr Sci, Rice Res Inst, Key Lab Rice Genet Breeding Anhui Prov, Hefei 230031, Peoples R China
2.Anhui Agr Univ, Coll Agron, Hefei 230036, Peoples R China
关键词： base editing; CRISPR; dual-base editor; genome editing; rice
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）
ISSN： 1672-9072
年卷期： 2021 年 63 卷 9 期
页码：
收录情况： SCI
摘要： An enhanced CDA-like (eCDAL) was established from Japanese lamprey CDA1-like 4 to achieve a high editing frequency in a broad region as a C-terminal cytosine base editors (CT-CBE). Then, a novel plant dual-base editor version 1(pDuBE1) was developed by integrating TadA-8e into eCDAL. The editing efficiency of pDuBE1 could reach to 87.6%, with frequencies of concurrent A-to-G and C-to-T conversions as high as 49.7% in stably transformed plant cells. Our results showed that pDuBE1 could mediate robust dual editing in plant genome, providing a powerful manipulation tool for precise crop breeding and screening platforms for in planta direct evolution.
- 相关文献
作者其他论文 更多>>
-
Conditional knockdown of OsMLH1 to improve plant prime editing systems without disturbing fertility in rice
作者：Liu, Xiaoshuang;Zhang, Yiru;Jiang, Yingli;Xiao, Zhi;Wei, Pengcheng;Gu, Dongfang;Xu, Rongfang;Qin, Ruiying;Li, Juan;Wei, Pengcheng
关键词：Prime editing; DNA mismatch repair; Rice; Fertility; Cre recombinase
-
Transcriptome Analysis Reveals Genes and Pathways Associated with Drought Tolerance of Early Stages in Sweet Potato (Ipomoea batatas (L.) Lam.)
作者：Cheng, Peng;Kong, Fanna;Han, Yang;Liu, Xiaoping;Xia, Jiaping
关键词：sweet potato; drought stress; transcriptome; RNA-seq; root; leaf
-
Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding
作者：Li, Yizhen;Liang, Jing;Deng, Bufang;Jiang, Yingli;Zhu, Jingyan;Chen, Like;Li, Min;Li, Juan
关键词：base editing; CRISPR; Cas9; plant; genome editing; crop improvement
-
An epi-allele of SMS causes Sanming dominant genic male sterility in rice
作者：Yang, Yachun;Li, Hao;Xu, Zuntao;Tai, Dewei;Ni, Dahu;Wei, Pengcheng;Yi, Chengxin;Yang, Jianbo;Yang, Yachun;Zhang, Cheng;Li, Hao;Xu, Zuntao;Tai, Dewei;Ding, Yong;Zhang, Cheng;Yang, Zeyuan;Xu, Zuntao;Ding, Yong
关键词：male sterility; rice; epi-allele; Sanming; DNA methylation
-
Development of plant cytosine base editors with the Cas12a system
作者：Wang, Huanhuan;Liang, Jing;Chen, Like;Deng, Bufang;Liu, Xiaoshuang;Zhu, Yitong;Zhao, Liangxia;Chen, Yanjun;Jiang, Yingli;Wei, Pengcheng;Gu, Dongfang;Jin, Shan;Xu, Rongfang;Qin, Ruiying;Kou, Dourong;Li, Juan
关键词：Cas12a; Base editing; Rice; ABE; CBE
-
Identification and functional analysis of ZmDLS associated with the response to biotic stress in maize
作者：Wang, Yiting;Zou, Jie;Li, Jiali;Fan, Honghong;Kong, Fanna;Xu, Dafeng;Li, Jiaxin;Yang, Huaying;Zhang, Lin;Li, Tingchun;Xu, Lina
关键词：maize; terpene synthase; corn borer bite; Fusarium graminearum attack; defense response; transcription factor
-
Physiological and metabolic responses to nitrogen availability of rice (Oryza sativa L.) cultivars with differ in nitrogen efficient
作者：Ruan, Xinmin;Dong, Zhaorong;Ruan, Xinmin;Du, Hongyang;Zhan, Xinchun;Cong, Xihan;Shi, Fuzhi;Luo, Zhixiang;Li, Juan
关键词：Rice; Nitrogen-use efficiency (NUE); Metabolome; Carbon metabolism; Tricarboxylic acid (TCA)
