文献类型： 外文期刊

作者： Chen, Xiaodong ¹ ; Sun, Hao ¹ ; Zhao, Bin ¹ ; Wang, Rui ¹ ; Ji, Changhao ¹ ; Zhu, Bin ¹ ;

作者机构： 1.Anhui Acad Agr Sci, Crop Res Inst, Hefei 230031, Peoples R China; Key Lab Crop Qual Improvement Anhui Prov, Hefei 230031, Peoples R China

关键词： Barley; Lignin biosynthesis; Differentially expressed genes

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 236 卷

页码：

收录情况： SCI

摘要： Lignin is an essential metabolite for plant growth but negatively affects the quality of forage barley. Genetic modification of quality traits to improve the forage digestibility requires an understanding of the molecular mechanism of lignin biosynthesis. RNA-Seq was used to quantify transcripts differentially expressed among leaf, stem and spike tissues from two barley genotypes. A total of 13,172 differentially expressed genes (DEGs) were identified, of which much more up-regulated DEGs were detected from the contrasting groups of leaf vs spike (LS) and stem vs spike (S-S), and down-regulated DEGs were dominant in the group of stem vs leaf (S-L). 47 DEGs were successfully annotated to the monolignol pathway and six of them were candidate genes regulating the lignin biosynthesis. The qRT-PCR assay verified the expression profiles of the six candidate genes. Among them, four genes might positively regulate the lignin biosynthesis during forage barley development in terms of the consistency of their expression levels and changes of lignin content among the tissues, while the other two genes may have the reverse effects. These findings provide target genes for further investigations on molecular regulatory mechanisms of lignin biosynthesis and genetic resources for improvement of forage quality in barley molecular breeding programme.