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Novel function of a putative TaCOBL ortholog associated with cold response

文献类型： 外文期刊

作者： Liu, Fang-Fang 1 ; Wan, Ying-Xiu 1 ; Cao, Wen-Xin 1 ; Zhang, Qi-Qi 1 ; Li, Yao 1 ; Li, Yan 1 ; Zhang, Ping-Zhi 1 ; Si, Hong-Qi 2 ;

作者机构： 1.Anhui Acad Agr Sci, Crop Res Inst, Anhui Key Lab Crop Qual Improvement, Hefei 230031, Peoples R China

2.Anhui Agr Univ, Sch Agron, Hefei 230036, Peoples R China

关键词： Wheat; TaCOBL; Cold resistance; Haplotypes; Functional markers

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.8； 五年影响因子：2.7 ）

ISSN： 0301-4851

年卷期： 2023 年 50 卷 5 期

页码：

收录情况： SCI

摘要： The plant COBRA protein family plays an important role in secondary cell wall biosynthesis and the orientation of cell expansion. The COBRA gene family has been well studied in Arabidopsis thaliana, maize, rice, etc., but no systematic studies were conducted in wheat. In this study, the full-length sequence of TaCOBLs was obtained by homology cloning from wheat, and a conserved motif analysis confirmed that TaCOBLs belonged to the COBRA protein family. qRT-PCR results showed that the TaCOBL transcripts were induced by abiotic stresses, including cold, drought, salinity, and abscisic acid (ABA). Two haplotypes of TaCOBL-5B (Hap5B-a and Hap5B-b), harboring one indel (----/TATA) in the 5 ' flanking region (- 550 bp), were found on chromosome 5BS. A co-dominant marker, Ta5BF/Ta5BR, was developed based on the polymorphism of the two TaCOBL-5B haplotypes. Significant correlations between the two TaCOBL-5B haplotypes and cold resistance were observed under four environmental conditions. Hap5B-a, a favored haplotype acquired during wheat polyploidization, may positively contribute to enhanced cold resistance in wheat. Based on the promoter activity analysis, the Hap5B-a promoter containing a TATA-box was more active than that of Hap5B-b without the TATA-box under low temperature. Our study provides valuable information indicating that the TaCOBL genes are associated with cold response in wheat.

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