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Analysis of protein kinase C (HcPKC) gene expression and single-nucleotide polymorphisms related to inner shell color traits in Hyriopsis cumingii

文献类型： 外文期刊

作者： Zhang, Mengying 1 ; Chen, Xiajun 1 ; Zhang, Jinpan 1 ; Guo, Baiying 1 ; Li, Jiale 1 ; Bai, Zhiyi 1 ;

作者机构： 1.Shanghai Ocean Univ, Minist Agr & Rural Affairs, Key Lab Freshwater Aquat Genet Resources, Shanghai 201306, Peoples R China

2.Shanghai Ocean Univ, Shanghai Collaborat Innovat Ctr Aquat Anim Breedi, Shanghai 201306, Peoples R China

3.Anhui Acad Agr Sci, Fisher Inst, Hefei, Peoples R China

4.Shanghai Ocean Univ, Shanghai Engn Res Ctr Aquaculture, Shanghai 201306, Peoples R China

关键词： Hyriopsis cumingii; Nacre color; HcPKC; SNP

期刊名称：BMC GENOMIC DATA

ISSN：

年卷期： 2022 年 23 卷 1 期

页码：

收录情况： SCI

摘要： Background Protein kinase C (PKC) is a multifunctional serine and PKC can phosphorylate serine residues in the cytoplasmic domain of tyrosinase, thereby regulating the activity of tyrosinase. Activated PKC is bound to the melanosome membrane, and unactivated PKC is free in the cytoplasm of melanocytes. In this study, we study the role of PKC gene in the melanin synthesis pathway and its effect on the color of the nacre of H. cumingii. Results In this study, a HcPKC gene in H. cumingii was cloned and its effects on melanin synthesis and nacre color were studied. HcPKC was expressed in both purple and white mussels, and the level of mRNA expression was higher in the purple mussels than in white mussels. Strong and specific mRNA signals were detected in the dorsal epithelial cells of the mantle pallial layer, indicating that HcPKC may be involved in nacre formation. After SNP association with inner shell color related traits, according to the principle that 0.25 < PIC < 0.5 is medium polymorphism and PIC < 0.25 is low polymorphism, the A + 332G site on the HcPKC gene was a site of moderate polymorphism, and the other four sites were low polymorphism sex sites. There was strong linkage disequilibrium among the five loci. A haplotype was constructed and it was found that the frequency of T1 (AGGAA)in the white population was significantly higher than that in the purple population (P < 0.05). Conclusion The study found that HcPKC of H. cumingii can be used as a candidate gene related to inner shell color, and some of the SNP sites can be used for molecular-assisted breeding in the spinnaker mussel, providing a reference for cultivating high-quality freshwater pearls.

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