Response of MaHMA2 gene expression and stress tolerance to zinc stress in mulberry (Morus alba L.)
文献类型： 外文期刊
作者： WANG, L. E., I 1 ; DU, Q. I. U. X. I. A. 1 ; SHI, Y. I. S. U. 1 ; ACKAH, M. I. C. H. A. E. L. 1 ; GUO, P. E. N. G. 1 ; ZHENG, D. A. N. Y. A. N. 1 ; WU, M. E. N. G. M. E. N. G. 1 ; JIN, X. I. N. 1 ; LI, P. E. I. L. A. N. 3 ; ZHANG, Q. I. A. O. N. A. N. 1 ; LI, R. U. I. X. U. E. 4 ; YIN, Z. H. I. 3 ; ZHAO, M. E. N. G. D., I 2 ; ZHAO, W. E. I. G. U. O. 1 ;
作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biol & Technol, Zhenjiang 212018, Jiangsu, Peoples R China
2.Suzhou Univ Sci & Technol, Dept Mat Sci & Engn, Suzhou 215004, Peoples R China
3.Nanjing Univ Finance & Econ, Nanjing 210023, Peoples R China
4.Anhui Acad Agr Sci, Sericultural Res Inst, Hefei 230061, Peoples R China
关键词： Mulberry; Zn stress; MaHMA2 gene; VGIS; Physiological; Biochemical
期刊名称：BIOCELL （ 影响因子：1.11； 五年影响因子：0.977 ）
ISSN： 0327-9545
年卷期： 2022 年 46 卷 10 期
页码：
收录情况： SCI
摘要： HMA2 (heavy metal ATPase 2) plays a crucial role in extracellular and intracellular Zn2+ transport across biomembranes, maintaining ion homeostasis, and playing an important role in the normal physiological metabolism, growth, and development of plants. In our study, a novel HMA2 gene, named MaHMA2, was isolated and cloned from white mulberry (Morus alba L.). The gene sequence obtained was 1,342 bp long, with an open reading frame of 1,194 bp, encoding a protein of 397 amino acids, with a predicted molecular mass of 42.852 kD and an isoelectric point of 7.53. This protein belonged to the PIB-type ATPase transport protein family. We analyzed the expression of the MaHMA2 gene by quantitative real-time PCR. The results showed that the level of MaHMA2 gene expression decreased to a Zn concentration of 800 mg/kg. Malondialdehyde and proline levels increased and responded to increasing Zn when the MaHMA2 gene was silenced, whereas the activities of peroxidase and superoxide dismutase tended to increase in response to increasing Zn2+ ion stress concentrations but were lower in the gene-silenced plants. These findings suggested that the MaHMA2 gene played an active role in the tolerance response of mulberry to Zn stress.
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