Biological Degradation of Aflatoxin B-1 by Cell-Free Extracts of Bacillus velezensis DY3108 with Broad PH Stability and Excellent Thermostability
文献类型： 外文期刊
作者： Shu, Xian 1 ; Wang, Yuting 1 ; Zhou, Qing 1 ; Li, Minghao 1 ; Hu, Hao 1 ; Ma, Yuhan 1 ; Chen, Xue 1 ; Ni, Jun 1 ; Zhao, Weiw 1 ;
作者机构： 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
3.Anhui Acad Agr Sci, Sericultural Res Inst, Hefei 230031, Anhui, Peoples R China
4.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Environm Toxicol & Pollut Control Technol, Hefei 230031, Anhui, Peoples R China
关键词： mycotoxin; aflatoxin B-1; Bacillus velezensis; biodegradation; detoxification
期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）
ISSN： 2072-6651
年卷期： 2018 年 10 卷 8 期
页码：
收录情况： SCI
摘要： (1) Background: Aflatoxin contamination in food and grain poses serious problems both for economic development and public health protection, thus leading to a focus on an effective approach to control it; (2) Methods: Aflatoxin B-1 (AFB(1)) degrading bacteria were isolated using a medium containing coumarin as the sole carbon source, and the biodegradation of AFB(1) by the isolate was examined by high performance liquid chromatography, and liquid chromatography mass spectrometry; (3) Results: a bacterial strain exhibiting strong AFB(1) degradation activity (91.5%) was isolated and identified as Bacillus velezensis DY3108. The AFB(1) degrading activity was predominantly attributed to the cell-free supernatant of strain DY3108. Besides, it was heat-stable and resistant to proteinase K treatment but sensitive to sodium dodecyl sulfate treatment. The optimal temperature for the maximal degradation of AFB(1) was 80 degrees C. Even more notable, the supernatant showed a high level of activity over a broad pH (4.0 to 11.0) and exhibited the highest degradation (94.70%) at pH 8.0. Cytotoxicity assays indicated that the degradation products displayed significantly (p < 0.05) lower cytotoxic effects than the parent AFB(1); (4) Conclusions: B. velezensis DY3108 might be a promising candidate for exploitation in AFB(1) detoxification and bioremediation in food and feed matrices.
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