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CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome

文献类型: 外文期刊

作者: Li Hao 1 ; Qin Ruiying 1 ; Liu Xiaoshuang 1 ; Liao Shengxiang 1 ; Xu Rongfang 1 ; Yang Jianbo 1 ; Wei Pengcheng 1 ;

作者机构: 1.Anhui Acad Agr Sci, Inst Rice Res, Key Lab Rice Genet & Breeding, Hefei 230031, Peoples R China

期刊名称:RICE SCIENCE ( 影响因子:3.333; 五年影响因子:4.226 )

ISSN: 1672-6308

年卷期: 2019 年 26 卷 2 期

页码:

收录情况: SCI

摘要: Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor (ABE)-nCas9 tool that induced targeted A.T to G.C point mutation of a key single nucleotide polymorphism site in an important agricultural gene. Combined with the modified single-guide RNA variant, our plant ABE tool can efficiently achieve adenine base editing in the rice genome.

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