文献类型： 外文期刊
作者： Chen, Xiaowu 1 ; Shen, Yawei 1 ; Wu, Minglin 2 ; Zhao, Jinliang 1 ;
作者机构： 1.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China
2.Anhui Acad Agr Sci, Fisheries Res Inst, 40 South Nongke Rd, Hefei 230000, Anhui, Peoples R China
3.Shanghai Ocean Univ, Shanghai Engn Res Ctr Aquaculture, Shanghai 201306, Peoples R China
4.Shanghai Collaborat Innovat Aquat Anim Genet & Br, Shanghai 201306, Peoples R China
关键词： Interferon; Interferon regulatory factor 3; Interferon-stimulated response element; Siniperca chuatsi; Thymus
期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）
ISSN： 0920-1742
年卷期： 2019 年 45 卷 1 期
页码：
收录情况： SCI
摘要： Interferon regulatory factors (IRFs) are transcription factors of the interferon (IFN)-inducible signaling pathway essential for host immunity against antimicrobial infection by virus and bacteria. Interferon regulatory factor 3 (IRF3) regulates the expression of IFNs and IFN-stimulated genes by binding to the IFN stimulatory response element (ISRE). In this study, we analyze the thymus transcriptome of the mandarin fish Siniperca chuatsi and report the functional analysis of Irf3 from the thymus as an emerging model of antiviral approaches. The predicted S. chuatsi IRF3 (Sc-Irf3) protein has 465 amino acid residues and evolutionarily conserved domains and is clustered in the IRF3 subfamily on a phylogenetic tree. Sc-Irf3 upon transgenic expression was mainly found in the cytoplasm through Western blot analysis and microscopy, but it translocated to the nucleus after polyinosinic:polycytidylic acid (ploly I:C) treatment. Endogenous Sc-irf3 RNA expression was detected in all eight adult organs examined. Importantly, Sc-irf3 RNA expression was significantly upregulated by ploly(I:C) treatment in the adult organs. Concurrently, reporter assays revealed that Sc-Irf3 increased the transcriptional activity of the ifn promoter, a minimal ISRE-containing promoter, and ifn promoter of mandarin fish. Therefore, Sc-Irf3 plays a major role in the IFN immune defense system against virus infection.
- 相关文献
作者其他论文 更多>>
-
Dietary supplementation of Astragalus fermentation products improves the growth performance, immunological characteristics, and disease resistance of crucian carp (Carassius auratus)
作者：Shi, Lisha;Xue, Mingyang;Xing, Yangyang;Xu, Chen;Jiang, Nan;Fan, Yuding;Chen, Jianwu;Liu, Wei;Zeng, Lingbing;Zhou, Yong;Shi, Lisha;Wu, Yeying;Wu, Minglin
关键词：fermentation; growth characteristics; disease resistance; crucian carp; Astragalus
-
Whole-genome resequencing of grass carp (Ctenopharyngodon idella) for genome-wide association study on GCRV resistance
作者：Yu, Chengchen;Jiang, Yuchen;Zhang, Chenyang;Gui, Lang;Xu, Xiaoyan;Li, Jiale;Shen, Yubang;Yu, Chengchen;Jiang, Yuchen;Zhang, Chenyang;Gui, Lang;Xu, Xiaoyan;Li, Jiale;Shen, Yubang;Wu, Minglin
关键词：Ctenopharyngodon idella; GCRV; GWAS; SNP; Whole-genome resequencing
-
Transcriptome Analysis of the Spleen Provides Insight into the Immune Regulation of GCRV Resistance in Grass Carp (Ctenopharyngodon idella)
作者：Yu, Chengchen;Jiang, Yuchen;Xu, Xiaoyan;Li, Jiale;Shen, Yubang;Yu, Chengchen;Jiang, Yuchen;Xu, Xiaoyan;Li, Jiale;Shen, Yubang;Wu, Minglin;Shen, Yubang
关键词：Grass carp; Transcriptome; GCRV; Disease; Immune
-
Anti-inflammatory and antioxidant properties of rice bran oil extract in copper sulfate-induced inflammation in zebrafish (Danio rerio)
作者：Liu, Naicheng;Zhang, Peng;Xue, Mingyang;Zhang, Mengjie;Xiao, Zidong;Xu, Chen;Fan, Yuding;Liu, Wei;Zhou, Yong;Liu, Naicheng;Zhang, Peng;Zhang, Mengjie;Zhang, Qinghua;Liu, Naicheng;Zhang, Peng;Zhang, Mengjie;Zhang, Qinghua;Wu, Yeying;Wu, Minglin
关键词：Anti-inflammatory; Antioxidation; CuSO4; Rice bran oil; Tocotrienols; Zebrafish
-
The SNP polymorphisms associated with WSSV-resistance of prophenoloxidase in red swamp crayfish (Procambarus clarkii) and its immune response against white spot syndrome virus (WSSV)
作者：Zhang, Long;Li, Yanhe;Ouyang, Haofeng;Shi, Ruixue;Li, Yanhe;Wu, Minglin
关键词：Procambarus clarkii; Prophenoloxidase; Single nucleotide polymorphism; White spot syndrome virus; Innate immune
-
Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I
作者：Zeng, Weiwei;Dong, Hanxu;Yang, Ying;Chen, Yanfeng;Li, Hua;Bergmannc, Sven M.;Wu, Minglin;Liu, Hong
关键词：grass carp reovirus; identification; virulence; complete genomic; recombinant
-
Gene expression profiling of DNA methyltransferase genes in Siniperca chuatsi based on transcriptome sequencing
作者：Zhou, Min;Zhao, Jinliang;Zhao, Ziwei;Chen, Xiaowu;Wu, Minglin
关键词：DNA methyltransferases; gene expression; Siniperca chuatsi
