文献类型： 外文期刊
作者： Yan, Dankan 1 ; Han, Kelei 2 ; Lu, Yuwen 2 ; Peng, Jiejun 2 ; Rao, Shaofei 2 ; Wu, Guanwei 2 ; Liu, Yong 4 ; Chen, Jianping 1 ; Zheng, Hongying 2 ; Yan, Fei 2 ;
作者机构： 1.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou, Peoples R China
2.Ningbo Univ, Inst Plant Virol, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China
3.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei, Peoples R China
4.Hunan Acad Agr Sci, Inst Plant Protect, Changsha, Peoples R China
关键词： cysteine; milk vetch dwarf virus; Nanovirus; U2 protein; viral suppressor of RNA silencing
期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.9； 五年影响因子：5.9 ）
ISSN： 1464-6722
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Nanoviruses have multipartite, circular, single-stranded DNA genomes and cause huge production losses in legumes and other crops. No viral suppressor of RNA silencing (VSR) has yet been reported from a member of the genus Nanovirus. Here, we demonstrate that the nanovirus U2 protein is a VSR. The U2 protein of milk vetch dwarf virus (MDV) suppressed the silencing of the green fluorescent protein (GFP) gene induced by single-stranded and double-stranded RNA, and the systemic spread of the GFP silencing signal. An electrophoretic mobility shift assay showed that the U2 protein was able to bind double-stranded 21-nucleotide small interfering RNA (siRNA). The cysteine residues at positions 43, 79 and 82 in the MDV U2 protein are critical to its nuclear localization, self-interaction and siRNA-binding ability, and were essential for its VSR activity. In addition, expression of the U2 protein via a potato virus X vector induced more severe necrosis symptoms in Nicotiana benthamiana leaves. The U2 proteins of other nanoviruses also acted as VSRs, and the three conserved cysteine residues were indispensable for their VSR activity.
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