文献类型： 外文期刊

作者： Zhao, Chang ¹ ; Jiang, Benzheng ¹ ; Yan, Weizhe ¹ ; Wang, Xichun ¹ ; Ding, Hongyan ² ; Xia, Cheng ³ ;

作者机构： 1.Anhui Agr Univ, Coll Anim Sci & Technol, Hefei, Peoples R China

2.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Anhui Prov Key Lab Livestock & Poultry Prod Safety, Hefei, Peoples R China

3.Heilongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing, Peoples R China

关键词： Subclinical ketosis; Adiponectin; Anestrus; Granulosa cell; PI3K-AKT

期刊名称：REPRODUCTIVE BIOLOGY （ 影响因子：2.5； 五年影响因子：2.3 ）

ISSN： 1642-431X

年卷期： 2024 年 24 卷 3 期

页码：

收录情况： SCI

摘要： In dairy cows, the occurrence of subclinical ketosis (SCK) is particularly high during early lactation. Previously, we documented alterations in the abundance of adiponectin (ADPN) in anestrus cows with SCK in comparison to cows in estrus. In the present study, 60 cows were divided into two groups: control (C, n = 30) and SCK (n = 30). Based on cow's estrus situation in two group at 55-60 days postpartum, 15 anestrus SCK cows and estrus cows were designated the SCK-A group and C-E group, respectively. The SCK-A group had downregulated serum and follicular fluid ADPN levels compared with the C-E group. The serum ADPN level was positively correlated with the insulin level and follicle growth rate, and there was a positive correlation between ADPN and glucose in the follicular fluid. Primary culture of dairy cow granulosa cells (GCs) was established to observe the effect of low glucose (Glu) and/or ADPN on GCs cyclins and proteins important for steroid synthesis. The results showed that the addition of 1 mu g/mL ADPN alleviated the negative effects of low Glu treatment on the proliferation of GCs and the expression of steroid secretion related protein proteins. Treatment with LY294002 (PI3K inhibitor) four experimental GCs groups: control (0 mu g/mL ADPN), 1 mu g/mL ADPN, LY294002 inhibitor, and 1 mu g/mL ADPN+LY294002. The results showed that ADPN promotes the secretion of steroid hormones by GCs through the PI3K-AKT. In summary, ADPN plays a crucial role in ameliorating postpartum anestrus in dairy cows with SCK.