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First evidence showing that Pepper vein yellows virus P4 protein is a movement protein

文献类型： 外文期刊

作者： Li, Sangsang 1 ; Su, Xianyan 2 ; Luo, Xiangwen 3 ; Zhang, Yu 1 ; Zhang, Deyong 1 ; Du, Jiao 3 ; Zhang, Zhanhong 3 ; OuYan 1 ;

作者机构： 1.Hunan Univ, Longping Branch, Changsha 410125, Peoples R China

2.Anhui Acad Agr Sci, Plant Protect Inst, Hefei 230001, Peoples R China

3.Hunan Acad Agr Sci, Hunan Plant Protect Inst, Key Lab Pest Management Hort Crop Hunan Prov, 726 Second Yuanda Rd, Changsha 410125, Hunan, Peoples R China

关键词： Pepper vein yellows virus; P4 protein; Plasmodesmata; Movement protein; Cell-to-cell movement

期刊名称：BMC MICROBIOLOGY （ 影响因子：3.605； 五年影响因子：4.283 ）

ISSN： 1471-2180

年卷期： 2020 年 20 卷 1 期

页码：

收录情况： SCI

摘要： Background Plant viruses move through plasmodesmata (PD) to infect new cells. To overcome the PD barrier, plant viruses have developed specific protein(s) to guide their genomic RNAs or DNAs to path through the PD. Results In the present study, we analyzed the function of Pepper vein yellows virus P4 protein. Our bioinformatic analysis using five commonly used algorithms showed that the P4 protein contains an transmembrane domain, encompassing the amino acid residue 117-138. The subcellular localization of P4 protein was found to target PD and form small punctates near walls. The P4 deletion mutant or the substitution mutant constructed by overlap PCR lost their function to produce punctates near the walls inside the fluorescent loci. The P4-YFP fusion was found to move from cell to cell in infiltrated leaves, and P4 could complement Cucumber mosaic virus movement protein deficiency mutant to move between cells. Conclusion Taking together, we consider that the P4 protein is a movement protein of Pepper vein yellows virus.

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