您好，欢迎访问安徽省农业科学院 机构知识库！

Expression and Functional Analysis of Hepcidin from Mandarin Fish (Siniperca chuatsi)

文献类型： 外文期刊

作者： Shen, Yawei 1 ; Zhao, Ziwei 1 ; Zhao, Jinliang 2 ; Chen, Xiaowu 3 ; Cao, Ming 4 ; Wu, Minglin 5 ;

作者机构： 1.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China

2.Shanghai Engn Res Ctr Aquaculture, Shanghai 201306, Peoples R China

3.Shanghai Collaborat Innovat Aquat Anim Genet & Br, Shanghai 201306, Peoples R China

4.Guangdong Prov Fishery Germplasm Conservat Ctr, Guangzhou 511400, Guangdong, Peoples R China

5.Anhui Acad Agr Sci, Fisheries Res Inst, Hefei 230000, Anhui, Peoples R China

关键词： mandarin fish; liver; hepcidin; iron balance; immune response; miR-19c-5p

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2019 年 20 卷 22 期

页码：

收录情况： SCI

摘要： Hepcidin is a liver-derived peptide hormone that is related to iron balance and immunity in humans. However, its function in Siniperca chuatsi has not been well elucidated. In this study, we analyzed the expression and function of the S. chuatsi hepcidin (Sc-hep) gene. Sc-hep was specifically expressed in the liver and appeared to be one of the most highly expressed genes in the liver. After spleen and kidney necrosis virus (ISKNV) infection and lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (Poly I:C) stimulation, the expression of Sc-hep in the liver increased by approximately 110, 6500, and 225 times, respectively. After ferrous sulfate (FS) injection, the expression of Sc-hep in the liver increased approximately 520-fold. We found that miR-19c-5p could inhibit Sc-hep expression. Five CpG dinucleotides distributed in the promoter region showed no differential methylation between the liver and the stomach, both presenting high methylation rates. After FS or LPS injection, the expression of three iron balance-related genes (FPN1, TFR1, and FTN) and five immune-related cytokine genes (IL-1 beta, IL8, TNF-alpha, TLR22, and SOCS3) significantly changed. These results indicate that Sc-hep participates in the regulation of iron balance and plays an important role in the immune system. Sc-hep increased approximately 52-fold when mandarin fish were domesticated with artificial diets. Sc-hep might be used as a real-time biomarker of mandarin fish liver because its expression markedly varies under different physiological conditions.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台