文献类型： 外文期刊
作者： Xu, Ruijie 1 ; Yu, Ding 3 ; Yang, Song 2 ; Ren, Qinghua 1 ; Luo, Jingfan 1 ; Ding, Zhien 1 ;
作者机构： 1.Anhui Agr Univ, Sch Tea & Food Sci & Technol, Hefei 230036, Anhui, Peoples R China
2.Anhui Acad Agr Sci, Inst Agroprod Proc, Hefei 230031, Anhui, Peoples R China
3.Anhui Med Univ, 1 Affiliated Hosp, Hefei 230022, Anhui, Peoples R China
4.BoZhou Univ, Bozhou 236800, Peoples R China
关键词： Auxin; Browning; Callus; Paclitaxel; Tissue culture; Torreya grandis
期刊名称：CURRENT TOPICS IN NUTRACEUTICAL RESEARCH （ 影响因子：0.416； 五年影响因子：0.536 ）
ISSN： 1540-7535
年卷期： 2019 年 17 卷 4 期
页码：
收录情况： SCI
摘要： To develop callus-derived tissue culture models for the production of secondary plant metabolites, conditions for callus induction from stems, needles and young buds of Torreya grandis were examined. The young buds were the best tissues for callus induction followed by stems and needles. While most needle and bud calluses turned brown and ceased growth by second subcultures, stem calluses exhibited rapid growth much longer. B5 medium supplemented with 2.0 mg/L naphthalene acetic acid, 0.5 mg/L kinetin, 20g/L sucrose, and 8 g/L agar was the best medium for callus induction from stems when incubated at 25 degrees C at 12 h light-dark cycle. These produced masses of friable calluses with a growth index of 4.7. However, callus subcultures maintained in darkness were generally of yellow-green color. There were some seasonal differences in callus induction with March being the best. The contents of paclitaxel in the calluses from needles, stems, and aril were 0.0212%, 0.0160%, and 0.0073%, respectively. However, the contents of baccatin III were 0.0034%, 0.0051%, and 0% in the calluses from needles, stems, and aril, respectively. In the suspension culture, B5 medium supplemented with 20 g/L sucrose on day 1 and 20 mg/L methyl jasmonate on day 7 showed improved cellular productivity resulting in the highest amount of paclitaxel (0.0257%) (2.842-fold higher than that of the untreated control) on day 14.
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