Expression Analysis of Odorant-Binding Protein Genes and Chemosensory Protein Genes in Anomala corpulenta Motschulsky (Coleoptera: Scarabaeidae)
文献类型： 外文期刊
作者： Chen, Haoliang 1 ; Qu, Mingjing 2 ; Ali, Farman 1 ; Lin, Lulu 1 ; Xie, Minghui 1 ; Zhang, Guangling 1 ; Su, Weihua 1 ;
作者机构： 1.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei 230031, Anhui, Peoples R China
2.Shandong Peanut Res Inst, Qingdao 266100, Shandong, Peoples R China
3.Abdul Wali Khan Univ, Dept Agr, Mardan 23200, Khyber Pakhtunk, Pakistan
关键词： Relative expression; RT-qPCR; reference genes; Anomala corpulenta
期刊名称：JOURNAL OF THE KANSAS ENTOMOLOGICAL SOCIETY （ 影响因子：0.281； 五年影响因子：0.445 ）
ISSN： 0022-8567
年卷期： 2019 年 92 卷 1 期
页码：
收录情况： SCI
摘要： Anomala corpulenta is an insect pest that is widely distributed in China, and it can cause large economic losses in agriculture, horticulture, and forestry. It is difficult to control because most of the lifecycle is underground. Trapping adults would be an environmentally sustainable way to reduce damage caused by this insect. Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are considered to be important chemodetection proteins. In this study, we investigate the expression of odorant-binding protein genes and chemosensory protein genes in different tissues of the adult and other developmental stages. The antenna had the highest expression levels for OBPs and CSPs of all tissues, except for OBP7 which was most highly expressed in the head without antenna. Besides the antenna, the head without antenna had the next highest expression levels for all OBP and CSP genes. The expression of OBP2, OBP3, OBP14, and CSP in the adult was higher than in other stages, while, OBP7 was lower than in other stages. Before estimating the expression of OBPs and CSPs, seven candidate reference genes were tested for stability among the tissues of adult and other development stages. GA PDH was the best reference gene in different tissues of the adult, and RPL13a was the best reference gene in different development stages. Our results provide a foundation for further research on the molecular aspects of chemical communication of this insect.
- 相关文献
作者其他论文 更多>>
-
Biocontrol of plant parasitic nematodes by bacteria and fungi: a multi-omics approach for the exploration of novel nematicides in sustainable agriculture
作者：Ayaz, Muhammad;Zhao, Jing-Tian;Yu, Qing;Yu, Jing-Wen;Wu, Wen-Cui;Huang, Wen-Kun;Ayaz, Muhammad;Zhao, Wei;Chi, Yuan-Kai;Qi, Ren-De;Ali, Qurban;Ali, Farman;Khan, Abdur Rashid
关键词：biocontrol agents; plant parasitic nematodes; multi-omics approaches; novel nematicides; sustainable agriculture
-
RpedOBP1 plays key roles in aggregation pheromones reception of the Riptortus pedestris
作者：Zhong, Yongzhi;Lin, Lulu;Zhao, Wei;Uddin, Md Kafil;Xie, Minghui;Chen, Haoliang;Tang, Rui;Wei, Shuang;Zhang, Feng;Uddin, Md Kafil
关键词：Aggregation pheromone; Diapause; Odorant binding protein
-
Bacterial and Fungal Biocontrol Agents for Plant Disease Protection: Journey from Lab to Field, Current Status, Challenges, and Global Perspectives
作者：Ayaz, Muhammad;Zhao, Wei;Chi, Yuan-Kai;Qi, Ren-De;Ayaz, Muhammad;Yu, Xi-Yue;Yu, Qing;Zhao, Jing-Tian;Yu, Jing-Wen;Huang, Wen-Kun;Li, Cai-Hong;Ali, Qurban;Shafiq, Muhammad;Shafiq, Muhammad;Ali, Farman
关键词：biocontrol; natural products; rhizosphere microbes; plant diseases; sustainable agriculture
-
PBP1 plays key roles in sex pheromone reception of the fall armyworm
作者：Zhong, Yongzhi;Xie, Minghui;Lin, Lulu;Su, Weihua;Xu, Lina;Chen, Haoliang;Di, Zhongjuan;Li, Fengqi;Chen, Jiaqi;Kong, Xiangxin;Tang, Rui;Zhang, Feng;Zhang, Feng
关键词：Spodoptera frugiperda; Sex pheromone; Pheromone binding protein
-
Transcriptome Analysis of Detoxification-Related Genes in Spodoptera frugiperda (Lepidoptera: Noctuidae)
作者：Chen, Haoliang;Xie, Minghui;Lin, Lulu;Zhong, Yongzhi;Su, Weihua;Zhang, Feng;Zhang, Feng
关键词：transcriptome; fall armyworm; differential expression unigene; pesticide detoxification
-
Transcriptome analysis of Holotrichia oblita reveals differentially expressed unigenes related to reproduction and development under different photoperiods
作者：Xie, Minghui;Zhong, Yongzhi;Lin, Lulu;Zhang, Guangling;Su, Weihua;Chen, Haoliang;Ni, WanLi;Qu, Mingjing
关键词：Transcriptome; Holotrichia oblita; Differentially expressed unigenes; Reproduction and development; Photoperiod
-
Comparative analyses of six cytochrome P450 genes and their roles in differential insecticide susceptibilities between the red flour beetle and the confused flour beetle
作者：Chen, Haoliang;Chen, Chengyu;Chen, Haoliang;Chen, Chengyu;Yu, Zhitao;Silver, Kristopher;Hu, Fei;Zhu, Kun Yan;Campbell, James F.;Arthur, Frank H.;Huang, Yong;Hu, Fei
关键词：Cytochrome P450; Insecticide susceptibility; RNA interference; Tribolium castaneum; Tribolium confusum
