文献类型： 外文期刊

作者： Yu, Ye ¹ ; Liu, Xiao-Jing ¹ ; Ma, Xiao ¹ ; Zhang, Zhong-Jie ¹ ; Wang, Tai-Chu ³ ; Sun, Fan ³ ; Hou, Cheng-Xiang ¹ ; Li, M ¹ ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Zhenjiang 212018, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang, Jiangsu, Peoples R China

3.Anhui Acad Agr Sci, Sericultural Res Inst, Hefei, Anhui, Peoples R China

关键词： Bombyx mori; CRISPR; Cas9; minute wing; palmitoyltransferase; promoter

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2020 年 27 卷 1 期

页码：

收录情况： SCI

摘要： The silkworm Bombyx mori is an important lepidopteran model insect in which many kinds of natural mutants have been identified. However, molecular mechanisms of most of these mutants remain to be explored. Here we report the identification of a gene Bm-app is responsible for the silkworm minute wing (mw) mutation which exhibits exceedingly small wings during pupal and adult stages. Compared with the wild type silkworm, relative messenger RNA expression of Bm-app is significantly decreased in the u11 mutant strain which shows mw phenotype. A 10 bp insertion in the putative promoter region of the Bm-app gene in mw mutant strain was identified and the dual luciferase assay revealed that this insertion decreased Bm-app promoter activity. Furthermore, clustered regularly interspaced short palindromic repeats/RNA-guided Cas9 nucleases-mediated depletion of the Bm-app induced similar wing defects which appeared in the mw mutant, demonstrating that Bm-app controls wing development in B. mori. Bm-app encodes a palmitoyltransferase and is responsible for the palmitoylation of selected cytoplasmic proteins, indicating that it is required for cell mitosis and growth during wing development. We also discuss the possibility that Bm-app regulates wing development through the Hippo signaling pathway in B. mori.